Renal tubular SGK1 deficiency causes impaired K+ excretion via loss of regulation of NEDD4-2/WNK1 and ENaC

Lama Al-Qusairi, Denis Basquin, Ankita Roy, Matteo Stifanelli, Renuga Devi Rajaram, Anne Debonneville, Izabela Nita, Marc Maillard, Johannes Loffing, Arohan R. Subramanya, Olivier Staub

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The stimulation of postprandial K+ clearance involves aldosterone-independent and -dependent mechanisms. In this context, serum- and glucocorticoidinduced kinase (SGK)1, a ubiquitously expressed kinase, is one of the primary aldosterone-induced proteins in the aldosterone-sensitive distal nephron. Germline inactivation of SGK1 suggests that this kinase is fundamental for K+ excretion under conditions of K+ load, but the specific role of renal SGK1 remains elusive. To avoid compensatory mechanisms that may occur during nephrogenesis, we used inducible, nephron-specific Sgk1Pax8/LC1 mice to assess the role of renal tubular SGK1 in K+ regulation. Under a standard diet, these animals exhibited normal K+ handling. When challenged by a high-K+ diet, they developed severe hyperkalemia accompanied by a defect in K+ excretion. Molecular analysis revealed reduced neural precursor cell expressed developmentally downregulated protein (NEDD)4-2 phosphorylation and total expression. γ-Epithelial Na+ channel (ENaC) expression and α/γENaC proteolytic processing were also decreased in mutant mice. Moreover, with no lysine kinase (WNK)1, which displayed in control mice punctuate staining in the distal convoluted tubule and diffuse distribution in the connecting tubule/cortical colleting duct, was diffused in the distal convoluted tubule and less expressed in the connecting tubule/collecting duct of SgkPax8/LC1 mice. Moreover, Ste20-related proline/alanine-rich kinase phosphorylation, and Na+-Cl+ cotransporter phosphorylation/apical localization were reduced in mutant mice. Consistent with the altered WNK1 expression, increased renal outer medullary K+ channel apical localization was observed. In conclusion, our data suggest that renal tubular SGK1 is important in the regulation of K+ excretion via the control of NEDD4-2, WNK1, and ENaC.

Original languageEnglish (US)
Pages (from-to)F330-F342
JournalAmerican Journal of Physiology - Renal Physiology
Issue number2
StatePublished - Aug 1 2016
Externally publishedYes


  • Aldosterone
  • Epithelial Na channel
  • Epithelial transport
  • Neural precursor cell expressed developmentally downregulated protein 4-2
  • Phosphorylation
  • Potassium
  • Serum- and glucocorticoid-induced kinase 1
  • Ubiquitylation
  • With no lysine kinase 1

ASJC Scopus subject areas

  • Physiology
  • Urology


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