Renal sodium and magnesium reabsorption are not coupled in a mouse model of Gordon syndrome

Wouter H. van Megen, Paul R. Grimm, Paul A. Welling, Jenny van der Wijst

Research output: Contribution to journalArticlepeer-review

Abstract

Active reabsorption of magnesium (Mg 2+ ) in the distal convoluted tubule (DCT) of the kidney is crucial for maintaining Mg 2+ homeostasis. Impaired activity of the Na + -Cl -cotransporter (NCC) has been associated with hypermagnesiuria and hypomagnesemia, while increased activity of NCC, as observed in patients with Gordon syndrome, is not associated with alterations in Mg 2+ balance. To further elucidate the possible interrelationship between NCC activity and renal Mg 2+ handling, plasma Mg 2+ levels and urinary excretion of sodium (Na + ) and Mg 2+ were measured in a mouse model of Gordon syndrome. In this model, DCT1-specific expression of a constitutively active mutant form of the NCC-phosphorylating kinase, SPAK (CA-SPAK), increases NCC activity and hydrochlorothiazide (HCTZ)-sensitive Na + reabsorption. These mice were normomagnesemic and HCTZ administration comparably reduced plasma Mg 2+ levels in CA-SPAK mice and control littermates. As inferred by the initial response to HCTZ, CA-SPAK mice exhibited greater NCC-dependent Na + reabsorption together with decreased Mg 2+ reabsorption, compared to controls. Following prolonged HCTZ administration (4 days), CA-SPAK mice exhibited higher urinary Mg 2+ excretion, while urinary Na + excretion decreased to levels observed in control animals. Surprisingly, CA-SPAK mice had unaltered renal expression of Trpm6, encoding the Mg 2+ -permeable channel TRPM6, or other magnesiotropic genes. In conclusion, CA-SPAK mice exhibit normomagnesemia, despite increased NCC activity and Na + reabsorption. Thus, Mg 2+ reabsorption is not coupled to increased thiazide-sensitive Na + reabsorption, suggesting a similar process explains normomagnesemia in Gordon syndrome. Further research is required to unravel the molecular underpinnings of this phenomenon and the more pronounced Mg 2+ excretion after prolonged HCTZ administration.

Original languageEnglish (US)
Article numbere13728
JournalPhysiological Reports
Volume6
Issue number14
DOIs
StatePublished - Jul 2018
Externally publishedYes

Keywords

  • Gordon syndrome
  • kidney
  • magnesium
  • sodium

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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