Renal expression and activity of the germinal center kinase SK2

Andrey V. Cybulsky, Tomoko Takano, Joan Papillon, Abdelkrim Khadir, Krikor Bijian, Chu Chun Chien, Charles E. Alpers, Hamid Rabb

Research output: Contribution to journalArticle

Abstract

Rat fetal kidney mRNA was analyzed by RT-PCR to identify protein kinases. This screening demonstrated expression of a protein kinase consistent with SK2, a group II germinal center kinase and homolog of human Ste20-like kinase (SLK). SK2 mRNA, protein expression, and kinase activity were increased in rat fetal kidney homogenates (embryonic days 17-21) compared with adult controls. In adult kidneys subjected to cross-clamping of the renal artery, followed by reperfusion, SK2 mRNA, protein expression, and kinase activity were increased compared with untreated contralateral controls. By immunohistochemistry, SK2 expression was evident mainly in the cytoplasm of tubular epithelial cells in fetal and adult kidneys. There was also some expression in developing and mature podocytes, but staining of the interstitium was negative. In cultured renal tubular epithelial cells, SK2 kinase activity was increased after incubation with serum, or after exposure to chemical anoxia plus reexposure to glucose. Stable overexpression of SLK reduced cell proliferation and increased apoptosis and exacerbated apoptosis and necrosis after chemical anoxia plus reexposure to glucose. Thus SK2 is a renal epithelial protein kinase whose expression and activity are increased during development and recovery from acute renal failure, where tubular epithelial regeneration may recapitulate developmental processes. The actions of SK2 appear to be antiproliferative and may facilitate cell injury.

Original languageEnglish (US)
Pages (from-to)F16-F25
JournalAmerican Journal of Physiology - Renal Physiology
Volume286
Issue number1 55-1
DOIs
StatePublished - Jan 2004

Keywords

  • Protein kinases
  • Renal development
  • Renal failure
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Urology

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  • Cite this

    Cybulsky, A. V., Takano, T., Papillon, J., Khadir, A., Bijian, K., Chien, C. C., Alpers, C. E., & Rabb, H. (2004). Renal expression and activity of the germinal center kinase SK2. American Journal of Physiology - Renal Physiology, 286(1 55-1), F16-F25. https://doi.org/10.1152/ajprenal.00144.2003