Renal enzymes in kidneys cells selected by D valine medium

S. F. Gilbert, Barbara R Migeon

Research output: Contribution to journalArticle

Abstract

The ability of D-valine medium to inhibit fibroblasts in cultures derived from kidneys of various mammals has enabled the selective proliferation of epithelial cells in the absence of fibroblast overgrowth. Studies of these selected epithelial cells have demonstrated the presence of D-amino acid oxidase, carbonic anhydrase, high levels of alkaline phosphatase and the renal specific pattern of lactate dehydrogenase. The presence of these renal enzymes suggests that the selected epithelial cells are of renal tubular origin and indicates that these differentiated functions are retained in cultured cells.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalJournal of Cellular Physiology
Volume92
Issue number2
StatePublished - 1977

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Valine
Fibroblasts
Kidney
Epithelial Cells
Enzymes
D-Amino-Acid Oxidase
Mammals
Carbonic Anhydrases
L-Lactate Dehydrogenase
Cell culture
Alkaline Phosphatase
Cells
Cultured Cells

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Renal enzymes in kidneys cells selected by D valine medium. / Gilbert, S. F.; Migeon, Barbara R.

In: Journal of Cellular Physiology, Vol. 92, No. 2, 1977, p. 161-168.

Research output: Contribution to journalArticle

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