Renal enzymes in kidneys cells selected by D valine medium

S. F. Gilbert, Barbara R Migeon

Research output: Contribution to journalArticle


The ability of D-valine medium to inhibit fibroblasts in cultures derived from kidneys of various mammals has enabled the selective proliferation of epithelial cells in the absence of fibroblast overgrowth. Studies of these selected epithelial cells have demonstrated the presence of D-amino acid oxidase, carbonic anhydrase, high levels of alkaline phosphatase and the renal specific pattern of lactate dehydrogenase. The presence of these renal enzymes suggests that the selected epithelial cells are of renal tubular origin and indicates that these differentiated functions are retained in cultured cells.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalJournal of Cellular Physiology
Issue number2
Publication statusPublished - 1977


ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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