Renal distribution and metabolism of [2H9]choline. A 2H NMR and MRI study.

John Eng, B. A. Berkowitz, R. S. Balaban

Research output: Contribution to journalArticle

Abstract

Trimethylamines are required as substrates in the biosynthesis of a number of important molecules in the cell. Herein, we describe the use of choline, deuterated in its 9 methyl positions, as an NMR label for following the distribution and metabolism of methyl groups after intravenous choline infusion. Deuterium (2H) NMR spectroscopy of the rabbit kidney in vivo revealed a linear uptake of infused choline that was directly proportional to the rate of infusion. The sensitivity limit for the spectroscopic studies in vivo was in the order of 100 microM for a 2 min data collection. After the infusion, 2H NMR imaging of the kidney in vivo demonstrated high trimethylamine concentrations in both the cortex and inner medulla but not in the outer medulla. The inner medullary fraction, however, was more labile to diuresis induced by furosemide. Companion high resolution 2H NMR studies of extracts revealed a cortex betaine/choline concentration ratio of 0.69 +/- 0.05 (mean +/- SEM, n = 3) before furosemide administration. Following furosemide infusion, the cortex betaine/choline concentration ratio was 3 +/- 1 (n = 6). Thus, 2H renal images following furosemide treatment can be interpreted as metabolic maps of betaine distribution. In addition, extraction studies revealed high concentrations of labelled choline and betaine in the liver. These data demonstrate that 2H-labelled choline is an effective marker of choline methyl metabolism in vivo and should provide a unique tool for the investigation of this important substrate.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalNMR in Biomedicine
Volume3
Issue number4
StatePublished - Aug 1990
Externally publishedYes

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Choline
Metabolism
Magnetic resonance imaging
Nuclear magnetic resonance
Kidney
Betaine
Furosemide
Deuterium
Diuresis
Biosynthesis
Substrates
Intravenous Infusions
Liver
Nuclear magnetic resonance spectroscopy
Labels
Magnetic Resonance Spectroscopy
Magnetic Resonance Imaging
Rabbits
Scanning electron microscopy
Molecules

ASJC Scopus subject areas

  • Biophysics

Cite this

Renal distribution and metabolism of [2H9]choline. A 2H NMR and MRI study. / Eng, John; Berkowitz, B. A.; Balaban, R. S.

In: NMR in Biomedicine, Vol. 3, No. 4, 08.1990, p. 173-177.

Research output: Contribution to journalArticle

Eng, J, Berkowitz, BA & Balaban, RS 1990, 'Renal distribution and metabolism of [2H9]choline. A 2H NMR and MRI study.', NMR in Biomedicine, vol. 3, no. 4, pp. 173-177.
Eng, John ; Berkowitz, B. A. ; Balaban, R. S. / Renal distribution and metabolism of [2H9]choline. A 2H NMR and MRI study. In: NMR in Biomedicine. 1990 ; Vol. 3, No. 4. pp. 173-177.
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