Removal of immune-stimulatory components from surfaces by plasma discharges

Marina Hasiwa, Ondřej Kylián, Thomas Hartung, Francois Rossi

Research output: Contribution to journalArticle

Abstract

Immune-stimulating microbiological components like lipopolysaccharide (LPS), lipoteichoic acid (LTA) and zymosan bound onto surfaces lead to severe problems when brought in contact with the organism via surgical instruments or implants. We have shown, in recent studies, that it is possible to detect different immune-stimulating components directly on the surface, via an indirect detection method, using human whole-blood and the monocyte reaction to measure the inflammatory mediator release (IL-1β) by ELISA. With regard to the inactivation of pyrogenic substances, we present a method based on the application of a low-pressure microwave plasma discharge working at low temperatures. We found a fast (10 s to a few minutes) removal rate of the immune-stimulating competence for LPS, LTA and zymosan. To mimic the bacterial cell-wall, LPS in combination with muramyl dipeptide was employed and the decreasing rate of the inflammatory signal did not differ from pure LPS.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalInnate Immunity
Volume14
Issue number2
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Lipopolysaccharides
Zymosan
Acetylmuramyl-Alanyl-Isoglutamine
Microwaves
Interleukin-1
Surgical Instruments
Mental Competency
Cell Wall
Monocytes
Enzyme-Linked Immunosorbent Assay
Pressure
Temperature
lipoteichoic acid

Keywords

  • Depyrogenation
  • Lipopolysaccharide (LPS)
  • Lipoteichoic acid (LTA)
  • Low-pressure microwave plasma discharge
  • Muramyl dipeptide (MDP)
  • Zymosan

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases
  • Medicine(all)

Cite this

Removal of immune-stimulatory components from surfaces by plasma discharges. / Hasiwa, Marina; Kylián, Ondřej; Hartung, Thomas; Rossi, Francois.

In: Innate Immunity, Vol. 14, No. 2, 2008, p. 89-97.

Research output: Contribution to journalArticle

Hasiwa, Marina ; Kylián, Ondřej ; Hartung, Thomas ; Rossi, Francois. / Removal of immune-stimulatory components from surfaces by plasma discharges. In: Innate Immunity. 2008 ; Vol. 14, No. 2. pp. 89-97.
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