Reliable method for detection of programmed cell death in yeast.

Xinchen Teng, J Marie Hardwick

Research output: Contribution to journalArticle

Abstract

Accumulating evidence suggests that yeasts are capable of undergoing programmed cell death (PCD) to benefit long-term survival of the species, and that yeast and mammals may share at least partially conserved PCD pathways. In our experience, mammalian apoptosis assays have not been readily applicable to yeast. Therefore, to take advantage of yeast as a genetic tool to study PCD, we developed a yeast cell death assay that can reliably reveal viability differences between wild-type strains and strains lacking the mitochondrial fission genes DNM1/Drp1 and FIS1, orthologs of mammalian cell death regulators. Cell viability following treatment with acetic acid is quantified by colony formation and vital dye (FUN1) staining to reproducibly detect dose-dependent, genetically programmed yeast cell death.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume559
DOIs
StatePublished - 2009

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Cell Death
Yeasts
Mitochondrial Dynamics
Mitochondrial Genes
Acetic Acid
Mammals
Cell Survival
Coloring Agents
Apoptosis
Staining and Labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Reliable method for detection of programmed cell death in yeast. / Teng, Xinchen; Hardwick, J Marie.

In: Methods in molecular biology (Clifton, N.J.), Vol. 559, 2009, p. 335-342.

Research output: Contribution to journalArticle

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