TY - JOUR
T1 - Reliable method for detection of programmed cell death in yeast.
AU - Teng, Xinchen
AU - Hardwick, J. Marie
PY - 2009
Y1 - 2009
N2 - Accumulating evidence suggests that yeasts are capable of undergoing programmed cell death (PCD) to benefit long-term survival of the species, and that yeast and mammals may share at least partially conserved PCD pathways. In our experience, mammalian apoptosis assays have not been readily applicable to yeast. Therefore, to take advantage of yeast as a genetic tool to study PCD, we developed a yeast cell death assay that can reliably reveal viability differences between wild-type strains and strains lacking the mitochondrial fission genes DNM1/Drp1 and FIS1, orthologs of mammalian cell death regulators. Cell viability following treatment with acetic acid is quantified by colony formation and vital dye (FUN1) staining to reproducibly detect dose-dependent, genetically programmed yeast cell death.
AB - Accumulating evidence suggests that yeasts are capable of undergoing programmed cell death (PCD) to benefit long-term survival of the species, and that yeast and mammals may share at least partially conserved PCD pathways. In our experience, mammalian apoptosis assays have not been readily applicable to yeast. Therefore, to take advantage of yeast as a genetic tool to study PCD, we developed a yeast cell death assay that can reliably reveal viability differences between wild-type strains and strains lacking the mitochondrial fission genes DNM1/Drp1 and FIS1, orthologs of mammalian cell death regulators. Cell viability following treatment with acetic acid is quantified by colony formation and vital dye (FUN1) staining to reproducibly detect dose-dependent, genetically programmed yeast cell death.
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U2 - 10.1007/978-1-60327-017-5_23
DO - 10.1007/978-1-60327-017-5_23
M3 - Article
C2 - 19609767
AN - SCOPUS:70349760987
SN - 1064-3745
VL - 559
SP - 335
EP - 342
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -