Release of elastase from purified human lung mast cells and basophils - Identification as a Hageman factor cleaving enzyme

Henry Louis Meier, Edward S. Schulman, Louis W. Heck, Donald Macglashan, Harold H. Newball, Allen P. Kaplan

Research output: Contribution to journalArticle

Abstract

Elastase, a serine protease, is capable of inducing severe lung destruction in experimental animal models. We now report that this proteinase exists preformed in neutrophil-free sonicates of purified human lung mast cells (>98% purity) and in circulating peripheral blood basophils (>97% purity). The elastase levels in both cell types (41-174 ng/106 cells) represents approximately 3-20% of those found in human neutrophils; both cell types released their elastase following anti-IgE and ionophore A23187 challenge. The apparent molecular size of the mast cell enzyme on Sephadex G-100 gel filtration, as well as its inhibition profile, was identical to that of purified human neutrophil elastase. This mast cell elastase is identical to our previously reported mast cell-derived Hageman factor cleaving activity. Mast cell-, basophil-, and neutrophil-derived elastases cleave Hageman factor into fragments of 52,000 and 28.000 Da; cleavage by all three enzymes is inhibited by preincubation with polyclonal antibodies directed against human neutrophil elastase.

Original languageEnglish (US)
Pages (from-to)295-308
Number of pages14
JournalInflammation
Volume13
Issue number3
DOIs
StatePublished - Jun 1989

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Factor XII
Basophils
Pancreatic Elastase
Mast Cells
Leukocyte Elastase
Lung
Enzymes
Neutrophils
Factor XIIa
Ionophores
Calcimycin
Serine Proteases
Gel Chromatography
Peptide Hydrolases
Animal Models
Antibodies

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Release of elastase from purified human lung mast cells and basophils - Identification as a Hageman factor cleaving enzyme. / Meier, Henry Louis; Schulman, Edward S.; Heck, Louis W.; Macglashan, Donald; Newball, Harold H.; Kaplan, Allen P.

In: Inflammation, Vol. 13, No. 3, 06.1989, p. 295-308.

Research output: Contribution to journalArticle

Meier, Henry Louis ; Schulman, Edward S. ; Heck, Louis W. ; Macglashan, Donald ; Newball, Harold H. ; Kaplan, Allen P. / Release of elastase from purified human lung mast cells and basophils - Identification as a Hageman factor cleaving enzyme. In: Inflammation. 1989 ; Vol. 13, No. 3. pp. 295-308.
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