Release of chromatin template restriction in rabbit spermatozoa

W. D.W. Heston; Barry R. Zirkin; Donald S. Coffey

doi:10.1016/0006-291X(75)90233-8

Release of chromatin template restriction in rabbit spermatozoa

W. D.W. Heston, Barry R. Zirkin, Donald S. Coffey

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The addition of the acidic polymers heparin or polyxanthylic acid to rabbit spermatozoa or sperm heads previously exposed to disulfide reducing agents released sperm DNA template restriction and stimulated high levels of incorporation of DNA precursor into DNA, as assayed with exogenous DNA polymerase. Incorporation did not occur in the presence of DNAase, or in the absence of magnesium ion, any of the four deoxyribonucleotides, or E. coli DNA polymerase. This represents the first report that spermatozoa can synthesize DNA in vitro.

Original language	English (US)
Pages (from-to)	162-168
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(75)90233-8
State	Published - May 5 1975
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(75)90233-8

Cite this

@article{59edec534460433b9e6e4edce7ade76e,

title = "Release of chromatin template restriction in rabbit spermatozoa",

abstract = "The addition of the acidic polymers heparin or polyxanthylic acid to rabbit spermatozoa or sperm heads previously exposed to disulfide reducing agents released sperm DNA template restriction and stimulated high levels of incorporation of DNA precursor into DNA, as assayed with exogenous DNA polymerase. Incorporation did not occur in the presence of DNAase, or in the absence of magnesium ion, any of the four deoxyribonucleotides, or E. coli DNA polymerase. This represents the first report that spermatozoa can synthesize DNA in vitro.",

author = "Heston, {W. D.W.} and Zirkin, {Barry R.} and Coffey, {Donald S.}",

note = "Funding Information: The authors are grateful for the excellent technical assistance of Adele Boison, D. S. Pivec and D. C. Irby. This work was supported by NSF grant GB-41316 and a sub-contract with The Program for Applied Research on Fertility Regulation (PARFR 51), University of Minnesota.",

year = "1975",

month = may,

day = "5",

doi = "10.1016/0006-291X(75)90233-8",

language = "English (US)",

volume = "64",

pages = "162--168",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Release of chromatin template restriction in rabbit spermatozoa

AU - Heston, W. D.W.

AU - Zirkin, Barry R.

AU - Coffey, Donald S.

N1 - Funding Information: The authors are grateful for the excellent technical assistance of Adele Boison, D. S. Pivec and D. C. Irby. This work was supported by NSF grant GB-41316 and a sub-contract with The Program for Applied Research on Fertility Regulation (PARFR 51), University of Minnesota.

PY - 1975/5/5

Y1 - 1975/5/5

N2 - The addition of the acidic polymers heparin or polyxanthylic acid to rabbit spermatozoa or sperm heads previously exposed to disulfide reducing agents released sperm DNA template restriction and stimulated high levels of incorporation of DNA precursor into DNA, as assayed with exogenous DNA polymerase. Incorporation did not occur in the presence of DNAase, or in the absence of magnesium ion, any of the four deoxyribonucleotides, or E. coli DNA polymerase. This represents the first report that spermatozoa can synthesize DNA in vitro.

AB - The addition of the acidic polymers heparin or polyxanthylic acid to rabbit spermatozoa or sperm heads previously exposed to disulfide reducing agents released sperm DNA template restriction and stimulated high levels of incorporation of DNA precursor into DNA, as assayed with exogenous DNA polymerase. Incorporation did not occur in the presence of DNAase, or in the absence of magnesium ion, any of the four deoxyribonucleotides, or E. coli DNA polymerase. This represents the first report that spermatozoa can synthesize DNA in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0016736456&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016736456&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(75)90233-8

DO - 10.1016/0006-291X(75)90233-8

M3 - Article

C2 - 1096874

AN - SCOPUS:0016736456

SN - 0006-291X

VL - 64

SP - 162

EP - 168

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Release of chromatin template restriction in rabbit spermatozoa

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this