The addition of the acidic polymers heparin or polyxanthylic acid to rabbit spermatozoa or sperm heads previously exposed to disulfide reducing agents released sperm DNA template restriction and stimulated high levels of incorporation of DNA precursor into DNA, as assayed with exogenous DNA polymerase. Incorporation did not occur in the presence of DNAase, or in the absence of magnesium ion, any of the four deoxyribonucleotides, or E. coli DNA polymerase. This represents the first report that spermatozoa can synthesize DNA in vitro.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 5 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology