Release of chromatin template restriction in rabbit spermatozoa

W. D.W. Heston, Barry R. Zirkin, Donald S. Coffey

Research output: Contribution to journalArticle

Abstract

The addition of the acidic polymers heparin or polyxanthylic acid to rabbit spermatozoa or sperm heads previously exposed to disulfide reducing agents released sperm DNA template restriction and stimulated high levels of incorporation of DNA precursor into DNA, as assayed with exogenous DNA polymerase. Incorporation did not occur in the presence of DNAase, or in the absence of magnesium ion, any of the four deoxyribonucleotides, or E. coli DNA polymerase. This represents the first report that spermatozoa can synthesize DNA in vitro.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume64
Issue number1
DOIs
StatePublished - May 5 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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