Release of arylsulfatase A but not B from rat mast cells by noncytolytic secretory stimuli

S. M. Lynch, K. F. Austen, S. I. Wasserman

Research output: Contribution to journalArticlepeer-review

Abstract

The net percentage of release of arylsulfatase activity from purified rat mast cells induced by rabbit anti-rat P(ab')2 was consistently only about 1/3 that of histamine. Isoelectric focusing of the released and residual arylsulfatase activities demonstrated specific release of the A type without B and a net percentage of immunologic release of arylsulfatase A equivalent to that of histamine. When the net percentage of histamine and arylsulfatase A release were nearly maximal (88 and 76%) in response to the calcium ionophore A23187, specific release of arylsulfatase B did not occur. Thus, arylsulfatase A and not B was associated with the secretory granule released from the rat mast cell by reversed anaphylaxis or the calcium ionophore. In contrast, subcellular fractionation of water-lysed mast cells yielded arylsulfatase B with the heparin- and chymase-containing granule fraction and arylsulfatase A in the aqueous fraction comprised of cell sap and granule water eluate. It may be that arylsulfatase B resides in a minor second granule, whereas arylsulfatase A is loosely associated with the predominant secretory granule of the rat mast cell.

Original languageEnglish (US)
Pages (from-to)1394-1399
Number of pages6
JournalJournal of Immunology
Volume121
Issue number4
StatePublished - Dec 1 1978
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Release of arylsulfatase A but not B from rat mast cells by noncytolytic secretory stimuli'. Together they form a unique fingerprint.

Cite this