Relaxant effects of benzodiazepines on isolated human umbilical arteries and veins

A. B. Elgoyhen, P. S. Lorenzo, R. P. Rothlin, D. Spacavento, [No Value] Adler-Graschinsky

Research output: Contribution to journalArticle

Abstract

1. Human umbilical arterial rings precontracted with 40 mM KCl were relaxed in a concentration-dependent manner (6-100 μM) by the benzodiazepines (BZDs) Ro 5-4864, diazepam and clonazepam. 2. Pre-incubation with the central BZD antagonist Ro 15-1788 (10 μM) for 10 min did not prevent the reductions in KCl-induced responses caused by different concentrations of either clonazepam or diazepam in the umbilical arteries. On the other hand, the putative peripheral BZD antagonist, PK 11195, was as potent as the peripheral agonist Ro 5-4864 in relaxing KCl-contracted arterial rings. 3. Contractions of the human umbilical rings induced by 1 μM 5-hydroxytryptamine (5-HT) were diminished by 6-100 μM Ro 5-4864 as well as by 6-100 μM diazepam. 4. Diazepam and Ro 5-4864 also induced relaxaton of human umbilical veins precontracted with 40 mM KCl. The latter effect was not modified by the removal of the endothelium. 5. It is concluded that BZDs interfere with responses elicited through the activation of either voltage-operated (KCl) or receptor-operated (5-HT) calcium channels in the human umbilical vasculature. Although the clinical significance of these in vitro studies is unknown, they should be taken into account whenever high doses of BZDs are administered at the end of pregnancy.

Original languageEnglish (US)
Pages (from-to)373-379
Number of pages7
JournalJournal of Autonomic Pharmacology
Volume13
Issue number6
StatePublished - 1993
Externally publishedYes

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Umbilical Veins
Umbilical Arteries
Benzodiazepines
Diazepam
Umbilicus
Clonazepam
Flumazenil
Serotonin Receptors
Calcium Channels
Endothelium
Serotonin
Pregnancy
4'-chlorodiazepam

ASJC Scopus subject areas

  • Pharmacology
  • Neuroscience(all)

Cite this

Elgoyhen, A. B., Lorenzo, P. S., Rothlin, R. P., Spacavento, D., & Adler-Graschinsky, N. V. (1993). Relaxant effects of benzodiazepines on isolated human umbilical arteries and veins. Journal of Autonomic Pharmacology, 13(6), 373-379.

Relaxant effects of benzodiazepines on isolated human umbilical arteries and veins. / Elgoyhen, A. B.; Lorenzo, P. S.; Rothlin, R. P.; Spacavento, D.; Adler-Graschinsky, [No Value].

In: Journal of Autonomic Pharmacology, Vol. 13, No. 6, 1993, p. 373-379.

Research output: Contribution to journalArticle

Elgoyhen, AB, Lorenzo, PS, Rothlin, RP, Spacavento, D & Adler-Graschinsky, NV 1993, 'Relaxant effects of benzodiazepines on isolated human umbilical arteries and veins', Journal of Autonomic Pharmacology, vol. 13, no. 6, pp. 373-379.
Elgoyhen AB, Lorenzo PS, Rothlin RP, Spacavento D, Adler-Graschinsky NV. Relaxant effects of benzodiazepines on isolated human umbilical arteries and veins. Journal of Autonomic Pharmacology. 1993;13(6):373-379.
Elgoyhen, A. B. ; Lorenzo, P. S. ; Rothlin, R. P. ; Spacavento, D. ; Adler-Graschinsky, [No Value]. / Relaxant effects of benzodiazepines on isolated human umbilical arteries and veins. In: Journal of Autonomic Pharmacology. 1993 ; Vol. 13, No. 6. pp. 373-379.
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