Relaxant effect of pharmacological interventions increasing heart cyclic AMP and its protein kinase.

H. E. Cingolani, L. Vittone, A. O. Grassi, M. I. Argel

Research output: Contribution to journalArticle

Abstract

After 1 min of treatment with different positive inotropic interventions, cyclic nucleotide levels (cAMP and cGMP) and cAMP-dependent protein kinase activity were determined in heart homogenates. Glucagon, norepinephrine, and isoproterenol increased cAMP from 0.503 +/- 0.025 pmol/mg wet weight to 1.051 +/- 0.099, 0.900 +/- 0.064, and 0.982 +/- 0.138, respectively. Simultaneously, cAMP-dependent protein kinase activity ratio rose from 0.21 +/- 0.02 to 0.45 +/- 0.04 with glucagon, 0.33 +/- 0.02 with norepinephrine, and 0.34 +/- 0.02 with isoproterenol. The ratio between maximal velocities of contraction and relaxation (+T/-T) was significantly decreased by these interventions, whereas time to peak tension (TTP) was shortened by norepinephrine and isoproterenol. High calcium, ouabain, and paired stimulation did not affect cAMP-dependent protein kinase activity and +T/-T. Our results suggest that inotropic interventions increasing cAMP levels might primarily affect intracellular mechanisms causing relaxation.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalAdvances in myocardiology
Volume3
StatePublished - 1982
Externally publishedYes

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Cyclic AMP-Dependent Protein Kinases
Isoproterenol
Cyclic AMP
Protein Kinases
Norepinephrine
Pharmacology
Glucagon
Cyclic GMP-Dependent Protein Kinases
Cyclic Nucleotides
Ouabain
Calcium
Weights and Measures

ASJC Scopus subject areas

  • Medicine(all)

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Relaxant effect of pharmacological interventions increasing heart cyclic AMP and its protein kinase. / Cingolani, H. E.; Vittone, L.; Grassi, A. O.; Argel, M. I.

In: Advances in myocardiology, Vol. 3, 1982, p. 67-75.

Research output: Contribution to journalArticle

Cingolani, H. E. ; Vittone, L. ; Grassi, A. O. ; Argel, M. I. / Relaxant effect of pharmacological interventions increasing heart cyclic AMP and its protein kinase. In: Advances in myocardiology. 1982 ; Vol. 3. pp. 67-75.
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