Abstract
Gel-exclusion chromatography coupled with HPLC instrumentation can be used to determine the molecular weight of highly purified sodium hyaluronate in solution. The method is very reproducible, precise, and rapid, and allows molecular weight determinations up to 2 million to be done in the presence of considerable impurities. This technique offers considerable advantages over traditional light-scattering, sedimentation equilibrium, and viscometry methods for molecular weight determinations, in that HPLC-gel exclusion is rapid and not subject to errors arising from impurities. Simultaneous with molecular weight measurements, sodium hyaluronate concentrations can be determined with a lower range of 0.1 to 0.3 mg/ml dependent upon the sensitivity of the refractive index-detecting system. Unlike the carbazole reaction, this technique is unaffected by low-molecular-weight impurities such as monosaccharides or substances with relative molecular weights less than 18,000.
Original language | English (US) |
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Pages (from-to) | 387-395 |
Number of pages | 9 |
Journal | Analytical biochemistry |
Volume | 147 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1985 |
Externally published | Yes |
Keywords
- HPLC
- arthritis
- degenerative joint disease
- hyaluronate
- mucopolysaccharide
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology