Relative microelastic mapping of living cells by atomic force microscopy

Emad A-Hassan, William F. Heinz, Matthew D. Antonik, Neill P. D'Costa, Soni Nageswaran, Cora Ann Schoenenberger, Jan H. Hoh

Research output: Contribution to journalArticle

Abstract

The spatial and temporal changes of the mechanical properties of living cells reflect complex underlying physiological processes. Following these changes should provide valuable insight into the biological importance of cellular mechanics and their regulation. The tip of an atomic force microscope (AFM) can be used to indent soft samples, and the force versus indentation measurement provides information about the local viscoelasticity. By collecting force-distance curves on a time scale where viscous contributions are small, the forces measured are dominated by the elastic properties of the sample. We have developed an experimental approach, using atomic force microscopy, called force integration to equal limits (FIEL) mapping, to produce robust, internally quantitative maps of relative elasticity. FIEL mapping has the advantage of essentially being independent of the tip-sample contact point and the cantilever spring constant. FIEL maps of living Madine-Darby canine kidney (MDCK) cells show that elasticity is uncoupled from topography and reveal a number of unexpected features. These results present a mode of high-resolution visualization in which the contrast is based on the mechanical properties of the sample.

Original languageEnglish (US)
Pages (from-to)1564-1578
Number of pages15
JournalBiophysical Journal
Volume74
Issue number3
StatePublished - Mar 1998

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Atomic Force Microscopy
Elasticity
Physiological Phenomena
Mechanics
Canidae
Kidney

ASJC Scopus subject areas

  • Biophysics

Cite this

A-Hassan, E., Heinz, W. F., Antonik, M. D., D'Costa, N. P., Nageswaran, S., Schoenenberger, C. A., & Hoh, J. H. (1998). Relative microelastic mapping of living cells by atomic force microscopy. Biophysical Journal, 74(3), 1564-1578.

Relative microelastic mapping of living cells by atomic force microscopy. / A-Hassan, Emad; Heinz, William F.; Antonik, Matthew D.; D'Costa, Neill P.; Nageswaran, Soni; Schoenenberger, Cora Ann; Hoh, Jan H.

In: Biophysical Journal, Vol. 74, No. 3, 03.1998, p. 1564-1578.

Research output: Contribution to journalArticle

A-Hassan, E, Heinz, WF, Antonik, MD, D'Costa, NP, Nageswaran, S, Schoenenberger, CA & Hoh, JH 1998, 'Relative microelastic mapping of living cells by atomic force microscopy', Biophysical Journal, vol. 74, no. 3, pp. 1564-1578.
A-Hassan E, Heinz WF, Antonik MD, D'Costa NP, Nageswaran S, Schoenenberger CA et al. Relative microelastic mapping of living cells by atomic force microscopy. Biophysical Journal. 1998 Mar;74(3):1564-1578.
A-Hassan, Emad ; Heinz, William F. ; Antonik, Matthew D. ; D'Costa, Neill P. ; Nageswaran, Soni ; Schoenenberger, Cora Ann ; Hoh, Jan H. / Relative microelastic mapping of living cells by atomic force microscopy. In: Biophysical Journal. 1998 ; Vol. 74, No. 3. pp. 1564-1578.
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