Relation of potassium transport to oxidative metabolism in isolated brain capillaries

Research output: Contribution to journalArticle

Abstract

The uptake of K by a capillary suspension isolated from rat brain was studied with the radioactive analogue 86Rb. Rb uptake was dependent upon the presence of oxygen and could be markedly inhibited with ouabain. The ouabain sensitive Rb uptake was measured at varying external concentrations of K. Uptake of K (as 86Rb) was half-maximal when the K concentration was 3-0 mM. This in vitro affinity of the transport carrier for K is similar to that found in previous in vivo studies of K efflux from brain to blood. I propose that the ouabain sensitive K pump is located on the antiluminal plasma membrane of brain capillary endothelial cells and that this pump contributes to the maintenance of a constant concentration (i.e. 3 mM) of K in brain interstitial fluid. Glucose and palmitate were tested as possible energy substrates for the support of active Rb uptake by isolated brain capillaries. The rate of Rb uptake increased 40% when 0.25 mM-palmitate was added to a capillary suspension containing 5 mM-glucose. This stimulation of Rb uptake could be blocked by 1 mM-4-pentenoic acid, an inhibitor of fatty acid oxidation. In contrast, the fraction of Rb uptake supported by glucose was not altered by 4-pentenoic acid. The rates of [U-14]palmitate oxidation to CO2 were measured in isolated brain capillaries and compared to their oxidation by brain slices and synaptosomes. Palmitate was the source of 28% of the 14CO2 produced by the capillaries but only 0.5% of the 14CO2 produced by the brain slices and synaptosomes. It is concluded that brain capillaries are similar to renal tubules in their polar distribution of ouabain sensitive K transport carriers, dependence on oxidative metabolism for active ion transport, and use of fatty acids as energy substrates. These features may underlie the vulnerability of brain capillaries in several metabolic diseases that cause brain oedema.

Original languageEnglish (US)
Pages (from-to)185-195
Number of pages11
JournalJournal of Physiology
VolumeVol. 286
StatePublished - 1979
Externally publishedYes

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Potassium
Brain
Palmitates
Ouabain
Synaptosomes
Glucose
Suspensions
Fatty Acids
Metabolic Diseases
Extracellular Fluid
Ion Transport
Brain Edema
Endothelial Cells
Maintenance
Cell Membrane
Oxygen
Kidney

ASJC Scopus subject areas

  • Physiology

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Relation of potassium transport to oxidative metabolism in isolated brain capillaries. / Goldstein, Gary William.

In: Journal of Physiology, Vol. Vol. 286, 1979, p. 185-195.

Research output: Contribution to journalArticle

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