Relation between glycolysis and calcium homeostasis in postischemic myocardium

Richmond W. Jeremy, Yukihiro Koretsune, Eduardo Marban, Lewis Becker

Research output: Contribution to journalArticle

Abstract

This study examined the hypothesis that glycolysis is required for functional recovery of the myocardium during reperfusion by facilitating restoration of calcium homeostasis. [Ca2+]i was measured in isolated perfused rabbit hearts by using the Ca2+ indicator 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetraacetic acid (5F-BAPTA) and 19F nuclear magnetic resonance spectroscopy. In nonischemic control hearts, inhibition of glycolysis with iodoacetate did not alter [Ca2+]i. In hearts subjected to 20 minutes of global zero-flow ischemia, [Ca2+]i increased from 260±80 nM before ischemia to 556±44 nM after 15 minutes of ischemia (p2+]i increased further in hearts with intact glycolysis to 851±134 nM (p2+]i, 1,380±260 nM after 15 minutes of reperfusion, po, 0.63 mM for 30 minutes, then 2.50 mM for 30 minutes). In these hearts, the rate/pressure product after 60 minutes of reperfusion was 12,492±1,561 mm Hg/min (po of 2.50 mM). These findings indicate that the functional impairment observed in postischemic myocardium is related to cellular Ca2+ overload. Glycolysis appears to play an important role in restoration of Ca2+ homeostasis and recovery of function of postischemic myocardium.

Original languageEnglish (US)
Pages (from-to)1180-1190
Number of pages11
JournalCirculation Research
Volume70
Issue number6
StatePublished - 1990

Fingerprint

Glycolysis
Myocardium
Homeostasis
Reperfusion
Calcium
Ischemia
Iodoacetates
Ethane
Recovery of Function
Magnetic Resonance Spectroscopy
Heart Rate
Rabbits
Pressure
Acids

Keywords

  • Calcium
  • Glycolysis
  • Myocardial ischemia
  • Reperfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Relation between glycolysis and calcium homeostasis in postischemic myocardium. / Jeremy, Richmond W.; Koretsune, Yukihiro; Marban, Eduardo; Becker, Lewis.

In: Circulation Research, Vol. 70, No. 6, 1990, p. 1180-1190.

Research output: Contribution to journalArticle

Jeremy, RW, Koretsune, Y, Marban, E & Becker, L 1990, 'Relation between glycolysis and calcium homeostasis in postischemic myocardium', Circulation Research, vol. 70, no. 6, pp. 1180-1190.
Jeremy, Richmond W. ; Koretsune, Yukihiro ; Marban, Eduardo ; Becker, Lewis. / Relation between glycolysis and calcium homeostasis in postischemic myocardium. In: Circulation Research. 1990 ; Vol. 70, No. 6. pp. 1180-1190.
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