Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis

Emily Kendall, Fahima Chowdhury, Yasmin Begum, Ashraful I. Khan, Shan Li, James H. Thierer, Jason Bailey, Kristen Kreisel, Carol O. Tacket, Regina C. LaRocque, Jason B. Harris, Edward T. Ryan, Firdausi Qadri, Stephen B. Calderwood, O. Colin Stine

Research output: Contribution to journalArticle

Abstract

The genetic relatedness of Vibrio cholerae O1/O139 isolates obtained from 100 patients and 146 of their household contacts in Dhaka, Bangladesh, between 2002 and 2005 was assessed by multilocus variable-number tandem-repeat analysis. Isolate genotypes were analyzed at five loci containing tandem repeats. Across the population, as well as within households, isolates with identical genotypes were clustered in time. Isolates from individuals within the same household were more likely to have similar or identical genotypes than were isolates from different households, but even within a household, isolates from different individuals often had different genotypes. When household contacts were sampled regularly for 3 weeks after the illness of the household index patient, isolates with genotypes related to the index patient appeared in contacts, on average, ∼3 days after the index patient, while isolates with unrelated genotypes appeared in contacts ∼6 days after. Limited data revealed that multiple isolates from the same individual collected within days of each other or even from a single stool sample may have identical, similar, or unrelated genotypes as well. Our results demonstrate that genetically related V. cholerae strains cluster in local outbreaks but also suggest that multiple distinct strains of V. cholerae O1 may circulate simultaneously within a household.

Original languageEnglish (US)
Pages (from-to)4367-4376
Number of pages10
JournalJournal of Bacteriology
Volume192
Issue number17
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint

Vibrio cholerae O139
Vibrio cholerae O1
Minisatellite Repeats
Genotype
Tandem Repeat Sequences
Vibrio cholerae
Bangladesh
Disease Outbreaks

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis. / Kendall, Emily; Chowdhury, Fahima; Begum, Yasmin; Khan, Ashraful I.; Li, Shan; Thierer, James H.; Bailey, Jason; Kreisel, Kristen; Tacket, Carol O.; LaRocque, Regina C.; Harris, Jason B.; Ryan, Edward T.; Qadri, Firdausi; Calderwood, Stephen B.; Stine, O. Colin.

In: Journal of Bacteriology, Vol. 192, No. 17, 09.2010, p. 4367-4376.

Research output: Contribution to journalArticle

Kendall, E, Chowdhury, F, Begum, Y, Khan, AI, Li, S, Thierer, JH, Bailey, J, Kreisel, K, Tacket, CO, LaRocque, RC, Harris, JB, Ryan, ET, Qadri, F, Calderwood, SB & Stine, OC 2010, 'Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis', Journal of Bacteriology, vol. 192, no. 17, pp. 4367-4376. https://doi.org/10.1128/JB.00698-10
Kendall, Emily ; Chowdhury, Fahima ; Begum, Yasmin ; Khan, Ashraful I. ; Li, Shan ; Thierer, James H. ; Bailey, Jason ; Kreisel, Kristen ; Tacket, Carol O. ; LaRocque, Regina C. ; Harris, Jason B. ; Ryan, Edward T. ; Qadri, Firdausi ; Calderwood, Stephen B. ; Stine, O. Colin. / Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis. In: Journal of Bacteriology. 2010 ; Vol. 192, No. 17. pp. 4367-4376.
@article{f956d7a9df58497baf1b3a8a0ee99525,
title = "Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis",
abstract = "The genetic relatedness of Vibrio cholerae O1/O139 isolates obtained from 100 patients and 146 of their household contacts in Dhaka, Bangladesh, between 2002 and 2005 was assessed by multilocus variable-number tandem-repeat analysis. Isolate genotypes were analyzed at five loci containing tandem repeats. Across the population, as well as within households, isolates with identical genotypes were clustered in time. Isolates from individuals within the same household were more likely to have similar or identical genotypes than were isolates from different households, but even within a household, isolates from different individuals often had different genotypes. When household contacts were sampled regularly for 3 weeks after the illness of the household index patient, isolates with genotypes related to the index patient appeared in contacts, on average, ∼3 days after the index patient, while isolates with unrelated genotypes appeared in contacts ∼6 days after. Limited data revealed that multiple isolates from the same individual collected within days of each other or even from a single stool sample may have identical, similar, or unrelated genotypes as well. Our results demonstrate that genetically related V. cholerae strains cluster in local outbreaks but also suggest that multiple distinct strains of V. cholerae O1 may circulate simultaneously within a household.",
author = "Emily Kendall and Fahima Chowdhury and Yasmin Begum and Khan, {Ashraful I.} and Shan Li and Thierer, {James H.} and Jason Bailey and Kristen Kreisel and Tacket, {Carol O.} and LaRocque, {Regina C.} and Harris, {Jason B.} and Ryan, {Edward T.} and Firdausi Qadri and Calderwood, {Stephen B.} and Stine, {O. Colin}",
year = "2010",
month = "9",
doi = "10.1128/JB.00698-10",
language = "English (US)",
volume = "192",
pages = "4367--4376",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "17",

}

TY - JOUR

T1 - Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis

AU - Kendall, Emily

AU - Chowdhury, Fahima

AU - Begum, Yasmin

AU - Khan, Ashraful I.

AU - Li, Shan

AU - Thierer, James H.

AU - Bailey, Jason

AU - Kreisel, Kristen

AU - Tacket, Carol O.

AU - LaRocque, Regina C.

AU - Harris, Jason B.

AU - Ryan, Edward T.

AU - Qadri, Firdausi

AU - Calderwood, Stephen B.

AU - Stine, O. Colin

PY - 2010/9

Y1 - 2010/9

N2 - The genetic relatedness of Vibrio cholerae O1/O139 isolates obtained from 100 patients and 146 of their household contacts in Dhaka, Bangladesh, between 2002 and 2005 was assessed by multilocus variable-number tandem-repeat analysis. Isolate genotypes were analyzed at five loci containing tandem repeats. Across the population, as well as within households, isolates with identical genotypes were clustered in time. Isolates from individuals within the same household were more likely to have similar or identical genotypes than were isolates from different households, but even within a household, isolates from different individuals often had different genotypes. When household contacts were sampled regularly for 3 weeks after the illness of the household index patient, isolates with genotypes related to the index patient appeared in contacts, on average, ∼3 days after the index patient, while isolates with unrelated genotypes appeared in contacts ∼6 days after. Limited data revealed that multiple isolates from the same individual collected within days of each other or even from a single stool sample may have identical, similar, or unrelated genotypes as well. Our results demonstrate that genetically related V. cholerae strains cluster in local outbreaks but also suggest that multiple distinct strains of V. cholerae O1 may circulate simultaneously within a household.

AB - The genetic relatedness of Vibrio cholerae O1/O139 isolates obtained from 100 patients and 146 of their household contacts in Dhaka, Bangladesh, between 2002 and 2005 was assessed by multilocus variable-number tandem-repeat analysis. Isolate genotypes were analyzed at five loci containing tandem repeats. Across the population, as well as within households, isolates with identical genotypes were clustered in time. Isolates from individuals within the same household were more likely to have similar or identical genotypes than were isolates from different households, but even within a household, isolates from different individuals often had different genotypes. When household contacts were sampled regularly for 3 weeks after the illness of the household index patient, isolates with genotypes related to the index patient appeared in contacts, on average, ∼3 days after the index patient, while isolates with unrelated genotypes appeared in contacts ∼6 days after. Limited data revealed that multiple isolates from the same individual collected within days of each other or even from a single stool sample may have identical, similar, or unrelated genotypes as well. Our results demonstrate that genetically related V. cholerae strains cluster in local outbreaks but also suggest that multiple distinct strains of V. cholerae O1 may circulate simultaneously within a household.

UR - http://www.scopus.com/inward/record.url?scp=77956518764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956518764&partnerID=8YFLogxK

U2 - 10.1128/JB.00698-10

DO - 10.1128/JB.00698-10

M3 - Article

C2 - 20585059

AN - SCOPUS:77956518764

VL - 192

SP - 4367

EP - 4376

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 17

ER -