Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNAi Met binding to the ribosome

Adesh K. Saini, Jagpreet S. Nanda, Jon R. Lorsch, Alan G. Hinnebusch

Research output: Contribution to journalArticle

Abstract

eIF1A is the eukaryotic ortholog of bacterial translation initiation factor IF1, but contains a helical domain and long unstructured N-terminal tail (NTT) and C-terminal tail (CTT) absent in IF1. Here, we identify elements in these accessory regions of eIF1A with dual functions in binding methionyl initiator tRNA (Met-tRNAiMet) to the ribosome and in selecting AUG codons. A pair of repeats in the eIF1A CTT, dubbed Scanning Enhancer 1 (SE 1) and SE2, was found to stimulate recruitment of Met-tRNAi Met in the ternary complex (TC) with eIF2·GTP and also to block initiation at UUG codons. In contrast, the NTT and segments of the helical domain are required for the elevated UUG initiation occurring in SE mutants, and both regions also impede TC recruitment. Remarkably, mutations in these latter elements, dubbed scanning inhibitors SI1 and SI2, reverse the defects in TC loading and UUG initiation conferred by SE substitutions, showing that the dual functions of SE elements in TC binding and UUG suppression are mechanistically linked. It appears that SE elements enhance TC binding in a conformation conducive to scanning but incompatible with initiation, whereas SI elements destabilize this conformation to enable full accommodation of Met-tRNAiMet in the P site for AUG selection.

Original languageEnglish (US)
Pages (from-to)97-110
Number of pages14
JournalGenes and Development
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2010

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Keywords

  • EIF2
  • EIf1A
  • Initiation
  • Initiator
  • Scanning
  • Translation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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