Regulation of yeast NRD1 expression by premature transcription termination

John T. Arigo, Kristina L. Carroll, Jessica M. Ames, Jeffry Lynn Corden

Research output: Contribution to journalArticle

Abstract

The yeast RNA binding proteins Nrd1 and Nab3 are required for termination of nonpolyadenylated transcripts from RNA polymerase (Pol) II-transcribed snRNA and snoRNA genes. In this paper, we show that NRD1 expression is regulated by Nrd1- and Nab3-directed premature termination. Sequences recognized by these proteins are present in NRD1 mRNA and are required for regulated expression. Chromatin immunoprecipitation and transcription run-on experiments show that, in wild-type cells, Pol II occupancy is high at the 5′ end of the NRD1 gene and decreases at the 3′ end. Mutation of Nrd1 and Nab3 binding sites within the NRD1 mRNA leads to a relative increase in Pol II occupancy of downstream sequences. We further show that NRD1 autoregulation involves components of the exosome and a newly discovered exosome-activating complex. Together, these results show that NRD1 is a eukaryotic cellular gene regulated through premature transcription termination.

Original languageEnglish (US)
Pages (from-to)641-651
Number of pages11
JournalMolecular Cell
Volume21
Issue number5
DOIs
StatePublished - Mar 3 2006

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Exosomes
Yeasts
Small Nucleolar RNA
Genes
Small Nuclear RNA
Messenger RNA
Fungal Proteins
RNA-Binding Proteins
RNA Polymerase II
Chromatin Immunoprecipitation
Homeostasis
Binding Sites
Mutation
Proteins

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Regulation of yeast NRD1 expression by premature transcription termination. / Arigo, John T.; Carroll, Kristina L.; Ames, Jessica M.; Corden, Jeffry Lynn.

In: Molecular Cell, Vol. 21, No. 5, 03.03.2006, p. 641-651.

Research output: Contribution to journalArticle

Arigo, John T. ; Carroll, Kristina L. ; Ames, Jessica M. ; Corden, Jeffry Lynn. / Regulation of yeast NRD1 expression by premature transcription termination. In: Molecular Cell. 2006 ; Vol. 21, No. 5. pp. 641-651.
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