Regulation of type III nitric oxide synthase gene expression by nitric oxide

L. J. Millalt, L. V. Ravichandraa, Roger A Johns

Research output: Contribution to journalArticle

Abstract

This study addressed the hypothesis that nitric oxide (NO) is able to regulate the expression of the Type III NO synthase (NOS) gene in bovine pulmonary endothelial cells (BPEC's). Monolayer cultures of BPEC's were transiently iransfected with a plasmid containing the complete 5' flanking region of the Type III NOS gene linked to the reporter gene luciferase. Following transfection, cells were incubated in the presence or absence of NO donors for 48 hours until harvesting for assay of luciferase activity. Both sodium nitroprusside (SNP) and 3-morpholinosydnonimine (SIN-1), at concentrations from 10-7- 10-4, caused a significant increase in reporter gene activity (SNP: 1.63-fold ±0.13, mean ±SD, n=3, p-5M SNP causes an increase in levels of Type III NOS mRNA and protein, determined by northern and western blot analysis. These results suggest that NO causes an increase in the level of Type HI NOS gene transcription in BPEC's, resulting in increased production of NOS mRNA and protein.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type III
Gene expression
Nitric Oxide Synthase
endothelial cells
nitric oxide
Nitric Oxide
Nitroprusside
Genes
lungs
luciferase
Endothelial cells
Gene Expression
reporter genes
gene expression
Endothelial Cells
cattle
Luciferases
Reporter Genes
Lung
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Regulation of type III nitric oxide synthase gene expression by nitric oxide. / Millalt, L. J.; Ravichandraa, L. V.; Johns, Roger A.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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