Regulation of the SK3 channel by microRNA-499 - Potential role in atrial fibrillation

Tian You Ling; Xiao Li Wang; Qiang Chai; Tin Wah Lau; Celeste M. Koestler; Soon J. Park; Richard C. Daly; Kevin L. Greason; Jin Jen; Li Qun Wu; Wei Feng Shen; Win Kuang Shen; Yong Mei Cha; Hon Chi Lee

doi:10.1016/j.hrthm.2013.03.005

Regulation of the SK3 channel by microRNA-499 - Potential role in atrial fibrillation

Tian You Ling, Xiao Li Wang, Qiang Chai, Tin Wah Lau, Celeste M. Koestler, Soon J. Park, Richard C. Daly, Kevin L. Greason, Jin Jen, Li Qun Wu, Wei Feng Shen, Win Kuang Shen, Yong Mei Cha, Hon Chi Lee

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

Background MicroRNAs are important regulators of gene expression, including those involving electrical remodeling in atrial fibrillation (AF). Recently, KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), was found to be strongly associated with AF. Objectives To evaluate the changes in atrial myocardial microRNAs in patients with permanent AF and to determine the role of microRNA on the regulation of cardiac SK3 expression. Methods Atrial tissue obtained during cardiac surgery from patients (4 sinus rhythm and 4 permanent AF) was analyzed by using microRNA arrays. Potential targets of microRNAs were predicted by using software programs. The effects of specific microRNAs on target gene expression were evaluated in HL-1 cells from a continuously proliferating mouse hyperplastic atrial cardiomyocyte cell line. Interactions between microRNAs and targets were further evaluated by using luciferase reporter assay and by using Argonaute pull-down assay. Results Twenty-one microRNAs showed significant (>2-fold) changes in AF. MicroRNA 499 (miR-499) was upregulated by 2.33-fold (P

Original language	English (US)
Pages (from-to)	1001-1009
Number of pages	9
Journal	Heart Rhythm
Volume	10
Issue number	7
DOIs	https://doi.org/10.1016/j.hrthm.2013.03.005
State	Published - Jul 2013
Externally published	Yes

Keywords

Atrial fibrillation
Electrical remodeling
MicroRNA
SK3 channel
Small-conductance calcium-activated potassium channel

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine
Physiology (medical)

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10.1016/j.hrthm.2013.03.005

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@article{a431a95548e4482aa9a2117cce22307f,

title = "Regulation of the SK3 channel by microRNA-499 - Potential role in atrial fibrillation",

abstract = "Background MicroRNAs are important regulators of gene expression, including those involving electrical remodeling in atrial fibrillation (AF). Recently, KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), was found to be strongly associated with AF. Objectives To evaluate the changes in atrial myocardial microRNAs in patients with permanent AF and to determine the role of microRNA on the regulation of cardiac SK3 expression. Methods Atrial tissue obtained during cardiac surgery from patients (4 sinus rhythm and 4 permanent AF) was analyzed by using microRNA arrays. Potential targets of microRNAs were predicted by using software programs. The effects of specific microRNAs on target gene expression were evaluated in HL-1 cells from a continuously proliferating mouse hyperplastic atrial cardiomyocyte cell line. Interactions between microRNAs and targets were further evaluated by using luciferase reporter assay and by using Argonaute pull-down assay. Results Twenty-one microRNAs showed significant (>2-fold) changes in AF. MicroRNA 499 (miR-499) was upregulated by 2.33-fold (P ",

keywords = "Atrial fibrillation, Electrical remodeling, MicroRNA, SK3 channel, Small-conductance calcium-activated potassium channel",

author = "Ling, {Tian You} and Wang, {Xiao Li} and Qiang Chai and Lau, {Tin Wah} and Koestler, {Celeste M.} and Park, {Soon J.} and Daly, {Richard C.} and Greason, {Kevin L.} and Jin Jen and Wu, {Li Qun} and Shen, {Wei Feng} and Shen, {Win Kuang} and Cha, {Yong Mei} and Lee, {Hon Chi}",

year = "2013",

month = jul,

doi = "10.1016/j.hrthm.2013.03.005",

language = "English (US)",

volume = "10",

pages = "1001--1009",

journal = "Heart Rhythm",

issn = "1547-5271",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Regulation of the SK3 channel by microRNA-499 - Potential role in atrial fibrillation

AU - Ling, Tian You

AU - Wang, Xiao Li

AU - Chai, Qiang

AU - Lau, Tin Wah

AU - Koestler, Celeste M.

AU - Park, Soon J.

AU - Daly, Richard C.

AU - Greason, Kevin L.

AU - Jen, Jin

AU - Wu, Li Qun

AU - Shen, Wei Feng

AU - Shen, Win Kuang

AU - Cha, Yong Mei

AU - Lee, Hon Chi

PY - 2013/7

Y1 - 2013/7

N2 - Background MicroRNAs are important regulators of gene expression, including those involving electrical remodeling in atrial fibrillation (AF). Recently, KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), was found to be strongly associated with AF. Objectives To evaluate the changes in atrial myocardial microRNAs in patients with permanent AF and to determine the role of microRNA on the regulation of cardiac SK3 expression. Methods Atrial tissue obtained during cardiac surgery from patients (4 sinus rhythm and 4 permanent AF) was analyzed by using microRNA arrays. Potential targets of microRNAs were predicted by using software programs. The effects of specific microRNAs on target gene expression were evaluated in HL-1 cells from a continuously proliferating mouse hyperplastic atrial cardiomyocyte cell line. Interactions between microRNAs and targets were further evaluated by using luciferase reporter assay and by using Argonaute pull-down assay. Results Twenty-one microRNAs showed significant (>2-fold) changes in AF. MicroRNA 499 (miR-499) was upregulated by 2.33-fold (P

AB - Background MicroRNAs are important regulators of gene expression, including those involving electrical remodeling in atrial fibrillation (AF). Recently, KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), was found to be strongly associated with AF. Objectives To evaluate the changes in atrial myocardial microRNAs in patients with permanent AF and to determine the role of microRNA on the regulation of cardiac SK3 expression. Methods Atrial tissue obtained during cardiac surgery from patients (4 sinus rhythm and 4 permanent AF) was analyzed by using microRNA arrays. Potential targets of microRNAs were predicted by using software programs. The effects of specific microRNAs on target gene expression were evaluated in HL-1 cells from a continuously proliferating mouse hyperplastic atrial cardiomyocyte cell line. Interactions between microRNAs and targets were further evaluated by using luciferase reporter assay and by using Argonaute pull-down assay. Results Twenty-one microRNAs showed significant (>2-fold) changes in AF. MicroRNA 499 (miR-499) was upregulated by 2.33-fold (P

KW - Atrial fibrillation

KW - Electrical remodeling

KW - MicroRNA

KW - SK3 channel

KW - Small-conductance calcium-activated potassium channel

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Regulation of the SK3 channel by microRNA-499 - Potential role in atrial fibrillation

Abstract

Keywords

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