Regulation of the NaV 1.5 cytoplasmic domain by calmodulin

Sandra B Gabelli, Agedi Boto, Victoria Halperin Kuhns, Mario Antonio Bianchet, Federica Farinelli, Srinivas Aripirala, Jesse Yoder, Jean Jakoncic, Gordon F. Tomaselli, Mario L Amzel

Research output: Contribution to journalArticle

Abstract

Voltage-gated sodium channels (Nav) underlie the rapid upstroke of action potentials in excitable tissues. Binding of channel-interactive proteins is essential for controlling fast and long-term inactivation. In the structure of the complex of the carboxy-terminal portion of Nav 1.5 (CTNav 1.5) with calmodulin (CaM)-Mg2+ reported here, both CaM lobes interact with the CTNav 1.5. On the basis of the differences between this structure and that of an inactivated complex, we propose that the structure reported here represents a non-inactivated state of the CTNav', that is, the state that is poised for activation. Electrophysiological characterization of mutants further supports the importance of the interactions identified in the structure. Isothermal titration calorimetry experiments show that CaM binds to CTNav 1.5 with high affinity. The results of this study provide unique insights into the physiological activation and the pathophysiology of Nav channels.

Original languageEnglish (US)
Article number5126
JournalNature Communications
Volume5
DOIs
StatePublished - 2014

Fingerprint

calmodulin
Calmodulin
Chemical activation
activation
Voltage-Gated Sodium Channels
Calorimetry
Titration
titration
lobes
deactivation
Action Potentials
affinity
heat measurement
Tissue
proteins
electric potential
Proteins
Experiments
interactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Regulation of the NaV 1.5 cytoplasmic domain by calmodulin. / Gabelli, Sandra B; Boto, Agedi; Kuhns, Victoria Halperin; Bianchet, Mario Antonio; Farinelli, Federica; Aripirala, Srinivas; Yoder, Jesse; Jakoncic, Jean; Tomaselli, Gordon F.; Amzel, Mario L.

In: Nature Communications, Vol. 5, 5126, 2014.

Research output: Contribution to journalArticle

Gabelli, SB, Boto, A, Kuhns, VH, Bianchet, MA, Farinelli, F, Aripirala, S, Yoder, J, Jakoncic, J, Tomaselli, GF & Amzel, ML 2014, 'Regulation of the NaV 1.5 cytoplasmic domain by calmodulin', Nature Communications, vol. 5, 5126. https://doi.org/10.1038/ncomms6126
Gabelli, Sandra B ; Boto, Agedi ; Kuhns, Victoria Halperin ; Bianchet, Mario Antonio ; Farinelli, Federica ; Aripirala, Srinivas ; Yoder, Jesse ; Jakoncic, Jean ; Tomaselli, Gordon F. ; Amzel, Mario L. / Regulation of the NaV 1.5 cytoplasmic domain by calmodulin. In: Nature Communications. 2014 ; Vol. 5.
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AU - Boto, Agedi

AU - Kuhns, Victoria Halperin

AU - Bianchet, Mario Antonio

AU - Farinelli, Federica

AU - Aripirala, Srinivas

AU - Yoder, Jesse

AU - Jakoncic, Jean

AU - Tomaselli, Gordon F.

AU - Amzel, Mario L

PY - 2014

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