Regulation of the CIC-2 lung epithelial chloride channel by glycosylation of SP1

Neeraj Vij, Pamela L. Zeitlin

Research output: Contribution to journalArticle

Abstract

Chloride channel-2 (CIC-2) is a pH- and voltage-activated chloride channel that is highly expressed in mammalian fetal airway epithelia during the period of maximal fluid secretion. A high level of luminal CIC-2 protein expression is maintained by the SP1 transcription factor until SP1 and CIC-2 decline rapidly at birth. Using fetal (prell-19) and adult (L2) rat lung Type 2 cell lines, we demonstrate that the active higher-molecular-weight 105-kD isoform of SP1 is phosphorylated and glycosylated. Exposure of either cell line to high-dose glutamine is sufficient to induce glycosylation of SP1 and to induce and maintain CIC-2. Exposure to tunicamycin to inhibit SP1 glycosylation reduces CIC-2 expression. We also demonstrate that in vivo CIC-2 expression is similarly regulated. SP1 from 6-wk-old murine lung (high CIC-2 expression) is hyperphosphorylated and hyperglycosylated compared with SP1 from 16-wk-old lung (low CIC-2 expression). Our results support the hypothesis that glycosylation of SP1 produces the 105-kD isoform of SP1 and is involved in regulating CIC-2 gene expression.

Original languageEnglish (US)
Pages (from-to)754-759
Number of pages6
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume34
Issue number6
DOIs
StatePublished - Jun 2006

Fingerprint

Glycosylation
Chloride Channels
Lung
Protein Isoforms
Cells
Fluids and Secretions
Tunicamycin
Cell Line
Glutamine
Gene expression
Rats
Transcription Factors
Epithelium
Molecular Weight
Molecular weight
Parturition

Keywords

  • Chloride channel
  • Cystic fibrosis
  • Lung development
  • Mouse
  • Transcription factor

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Regulation of the CIC-2 lung epithelial chloride channel by glycosylation of SP1. / Vij, Neeraj; Zeitlin, Pamela L.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 34, No. 6, 06.2006, p. 754-759.

Research output: Contribution to journalArticle

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