Regulation of synapse structure and function by distinct myosin II motors

Maria D. Rubio, Richard Johnson, Courtney A. Miller, Richard L Huganir, Gavin Rumbaugh

Research output: Contribution to journalArticle

Abstract

Ongoing synaptic function and rapid, bidirectional plasticity are both controlled by regulatory mechanisms within dendritic spines. Spine actin dynamics maintain synapse structure and function, and cytoskeletal rearrangements in these structures trigger structural and functional plasticity. Therefore, proteins that interact with actin filaments are attractive candidates to regulate synaptic actin dynamics and, thus, synapse structure and function. Here, we have cloned the rat isoform of class II myosin heavy chain MyH7B in brain. Unexpectedly, this isoform resembles muscle-type myosin II rather than the ubiquitously expressed nonmuscle myosin II isoforms, suggesting that a rich functional diversity of myosin II motors may exist in neurons. Indeed, reducing the expression of MyH7B in mature neurons caused profound alterations to dendritic spine structure and excitatory synaptic strength. Structurally, dendritic spines had large, irregularly shaped heads that contained many filopodia-like protrusions. Neurons with reduced MyH7B expression also had impaired miniature EPSC amplitudes accompanied by a decrease in synaptic AMPA receptors, which was linked to alterations of the actin cytoskeleton. MyH7B-mediated control over spine morphology and synaptic strength was distinct from that of a nonmuscle myosin, myosin IIb. Interestingly, when myosin IIb expression and MyH7B expression were simultaneously knocked-down in neurons, a third, more pronounced phenotype emerged. Together, our data provide evidence that distinct myosin II isoforms work together to regulate synapse structure and function in cultured hippocampal neurons. Thus, myosin II motor activity is emerging as a broad regulatory mechanism for control over complex actin networks within dendritic spines.

Original languageEnglish (US)
Pages (from-to)1448-1460
Number of pages13
JournalJournal of Neuroscience
Volume31
Issue number4
DOIs
StatePublished - Jan 26 2011

Fingerprint

Myosin Type II
Dendritic Spines
Synapses
Protein Isoforms
Neurons
Myosins
Actins
Actin Cytoskeleton
Spine
Neurotransmitter Receptor
Pseudopodia
AMPA Receptors
Myosin Heavy Chains
Motor Activity
Head
Phenotype
Muscles
Brain
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regulation of synapse structure and function by distinct myosin II motors. / Rubio, Maria D.; Johnson, Richard; Miller, Courtney A.; Huganir, Richard L; Rumbaugh, Gavin.

In: Journal of Neuroscience, Vol. 31, No. 4, 26.01.2011, p. 1448-1460.

Research output: Contribution to journalArticle

Rubio, Maria D. ; Johnson, Richard ; Miller, Courtney A. ; Huganir, Richard L ; Rumbaugh, Gavin. / Regulation of synapse structure and function by distinct myosin II motors. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 4. pp. 1448-1460.
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