Regulation of plasminogen activator activity in human fibroblastic cells by fibrosarcoma cell-derived factors

O. Saksela, Marikki Laiho, J. Keski-Oja

Research output: Contribution to journalArticle

Abstract

Low-molecular-weight protein factors (M(r) 8,000 to 18,000) from serum-free conditioned medium of human fibrosarcoma (8387) cells reversibly enhanced the secretion of proteinase-inhibitory activity by cultured normal human skin fibroblasts. This inhibitory activity could be absorbed by immobilized plasminogen activator (PA) of urokinase type but not by heparin, and it was sensitive to treatment with sodium dodecyl sulfate. The secretion of a heparin-binding M(r) 60,000 proteinase inhibitor, resembling protease nexin, was also detected. Early passages of adult skin fibroblasts do not contain or secrete PA. When cell types secreting this enzyme were tested, the fibrosarcoma-derived factors decreased the PA secretion detectable after sodium dodecyl sulfate treatment in all conditioned media of normal and malignant fibroblastic cells examined, including the 8387 cell line itself. However, no effects on the secretion of PA by normal or malignant cells of epithelioid origin or by melanoma cells were seen. A similar preparation from human epidermoid carcinoma (A431)-conditioned medium did not affect the PA activity or secretion of proteinase inhibitors from fibroblastic cells. The ability of sarcoma cells to modulate the production of PA inhibitors is a novel characteristic in the regulation of cellular proteolysis.

Original languageEnglish (US)
Pages (from-to)2314-2319
Number of pages6
JournalCancer Research
Volume45
Issue number5
StatePublished - 1985
Externally publishedYes

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Fibrosarcoma
Plasminogen Activators
Human Activities
Conditioned Culture Medium
Peptide Hydrolases
Sodium Dodecyl Sulfate
Heparin
Protease Nexins
Fibroblasts
Plasminogen Inactivators
Epithelioid Cells
Skin
Serum-Free Culture Media
Urokinase-Type Plasminogen Activator
Sarcoma
Proteolysis
Squamous Cell Carcinoma
Melanoma
Molecular Weight
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Regulation of plasminogen activator activity in human fibroblastic cells by fibrosarcoma cell-derived factors. / Saksela, O.; Laiho, Marikki; Keski-Oja, J.

In: Cancer Research, Vol. 45, No. 5, 1985, p. 2314-2319.

Research output: Contribution to journalArticle

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