Regulation of luteinizing hormone receptor mRNA expression by a specific RNA binding protein in the ovary

K. M.J. Menon, Anil K. Nair, Lei Wang, Helle Peegel

Research output: Contribution to journalArticle

Abstract

The expression of LH receptor mRNA shows significant changes during different physiological states of the ovary. Previous studies from our laboratory have identified a post-transcriptional mechanism by which LH receptor mRNA is regulated following preovulatory LH surge or in response to hCG administration. A specific binding protein, identified as mevalonate kinase, binds to the open reading frame of LH receptor mRNA. The protein binding site is localized to nucleotides 203-220 of the LH receptor mRNA and exhibits a high degree of specificity. The expression levels of the protein show an inverse relationship to the LH receptor mRNA levels. The hCG-induced down-regulation of LH receptor mRNA can be mimicked by increasing the intracellular levels of cyclic AMP by a phosphodiesterase inhibitor. An in vitro mRNA decay assay showed that addition of the binding protein to the decay system caused accelerated LH receptor mRNA decay. Our results therefore show that LH receptor mRNA expression in the ovary is regulated post-transcriptionally by altering the rate of mRNA degradation by a specific mRNA binding protein.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume260-262
DOIs
StatePublished - Jan 2 2007
Externally publishedYes

Keywords

  • LHR mRNA
  • Mevalonate kinase
  • Post-transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Regulation of luteinizing hormone receptor mRNA expression by a specific RNA binding protein in the ovary'. Together they form a unique fingerprint.

  • Cite this