Regulation of ketone body production from [14C]palmitate in rat liver mitochondria: Effects of cyclic nucleotides and unlabeled fatty acids

John M. Amatruda, Simeon Margolis, Dean H. Lockwood

Research output: Contribution to journalArticlepeer-review

Abstract

N6′, O2′-dibutyryl adenosine 3′, 5′-cyclic monophosphoric acid, but not other cyclic nucleotides stimulates [14C]ketone body production from [14C]palmitate in isolated rat liver mitochondria. Butyrate alone, as well as unlabeled acetate, octanoate and palmitate had similar effects. This redistribution of the oxidative products of [14C]palmitate can best be explained by exceeding the capacity of the Krebs cycle and/or changes in the acetyl coenzyme A/coenzyme A ratio. In contrast to [14C]palmitate, [14C]octanoate oxidation to [14C]O2 and [14C]ketone bodies was inhibited by the addition of unlabeled fatty acids. This suggests that an additional mechanism by which unlabeled fatty acids may stimulate [14C]ketone body production is by enhancing the carnitine-dependent transport of [14C]palmitate into mitochondria.

Original languageEnglish (US)
Pages (from-to)1337-1345
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume67
Issue number4
DOIs
StatePublished - Dec 15 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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