TY - JOUR
T1 - Regulation of ketone body production from [14C]palmitate in rat liver mitochondria
T2 - Effects of cyclic nucleotides and unlabeled fatty acids
AU - Amatruda, John M.
AU - Margolis, Simeon
AU - Lockwood, Dean H.
N1 - Funding Information:
ACKNOWLEDGEMENTS. We are indebted to Drs. M. Daniel Lane and Lutz A. Kiesow for helpful discussions during the course of this work. These studies were supported by NIAMD Grants AM-13562 and AM-02202, NHLI Grant HC-15930 and a grant from the Weight Watchers Foundation, Inc.
PY - 1975/12/15
Y1 - 1975/12/15
N2 - N6′, O2′-dibutyryl adenosine 3′, 5′-cyclic monophosphoric acid, but not other cyclic nucleotides stimulates [14C]ketone body production from [14C]palmitate in isolated rat liver mitochondria. Butyrate alone, as well as unlabeled acetate, octanoate and palmitate had similar effects. This redistribution of the oxidative products of [14C]palmitate can best be explained by exceeding the capacity of the Krebs cycle and/or changes in the acetyl coenzyme A/coenzyme A ratio. In contrast to [14C]palmitate, [14C]octanoate oxidation to [14C]O2 and [14C]ketone bodies was inhibited by the addition of unlabeled fatty acids. This suggests that an additional mechanism by which unlabeled fatty acids may stimulate [14C]ketone body production is by enhancing the carnitine-dependent transport of [14C]palmitate into mitochondria.
AB - N6′, O2′-dibutyryl adenosine 3′, 5′-cyclic monophosphoric acid, but not other cyclic nucleotides stimulates [14C]ketone body production from [14C]palmitate in isolated rat liver mitochondria. Butyrate alone, as well as unlabeled acetate, octanoate and palmitate had similar effects. This redistribution of the oxidative products of [14C]palmitate can best be explained by exceeding the capacity of the Krebs cycle and/or changes in the acetyl coenzyme A/coenzyme A ratio. In contrast to [14C]palmitate, [14C]octanoate oxidation to [14C]O2 and [14C]ketone bodies was inhibited by the addition of unlabeled fatty acids. This suggests that an additional mechanism by which unlabeled fatty acids may stimulate [14C]ketone body production is by enhancing the carnitine-dependent transport of [14C]palmitate into mitochondria.
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U2 - 10.1016/0006-291X(75)90174-6
DO - 10.1016/0006-291X(75)90174-6
M3 - Article
C2 - 173341
AN - SCOPUS:0016794131
SN - 0006-291X
VL - 67
SP - 1337
EP - 1345
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -