TY - JOUR
T1 - Regulation of invasive cell behavior by taiman, a Drosophila protein related to AIB1, a steroid receptor coactivator amplified in breast cancer
AU - Bai, Jianwu
AU - Uehara, Yoshihiko
AU - Montell, Denise J.
N1 - Funding Information:
We wish to thank Rachael Fiske, Laura Hart, and Henry Pak for their help in generating and screening mutant lines. Eric Baehrecke, Greg Guild, Lynn Cooley, Ron Evans, Catherine Thompson, and Carl Thummel generously supplied plasmids and antibodies. We also thank Drs. Craig Montell and Hong-Sheng Li for important discussions and critical reading of the manuscript. This work was supported by grants to D. J. M. from the National Institutes of Health (R01 GM46425) and the American Cancer Society (RSG1487).
PY - 2000/12/22
Y1 - 2000/12/22
N2 - Steroid hormones are key regulators of numerous physiological and developmental processes, including metastasis of breast and ovarian cancer. Here we report the identification of a Drosophila gene, named taiman, which encodes a steroid hormone receptor coactivator related to AIB1. Mutations in tai caused defects in the migration of specific follicle cells, the border cells, in the Drosophila ovary. Mutant cells exhibited abnormal accumulation of E-cadherin, β-catenin, and focal adhesion kinase. TAI protein colocalized with the ecdysone receptor in vivo and augmented transcriptional activation by the ecdysone receptor in cultured cells. The finding of this type of coactivator required for cell motility suggests a novel role for steroid hormones, in stimulating invasive cell behavior, independent of effects on proliferation.
AB - Steroid hormones are key regulators of numerous physiological and developmental processes, including metastasis of breast and ovarian cancer. Here we report the identification of a Drosophila gene, named taiman, which encodes a steroid hormone receptor coactivator related to AIB1. Mutations in tai caused defects in the migration of specific follicle cells, the border cells, in the Drosophila ovary. Mutant cells exhibited abnormal accumulation of E-cadherin, β-catenin, and focal adhesion kinase. TAI protein colocalized with the ecdysone receptor in vivo and augmented transcriptional activation by the ecdysone receptor in cultured cells. The finding of this type of coactivator required for cell motility suggests a novel role for steroid hormones, in stimulating invasive cell behavior, independent of effects on proliferation.
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U2 - 10.1016/S0092-8674(00)00208-7
DO - 10.1016/S0092-8674(00)00208-7
M3 - Article
C2 - 11163181
AN - SCOPUS:0034704202
VL - 103
SP - 1047
EP - 1058
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -