Regulation of intracellular chloride activity during perfusion with hypertonic solutions in the Necturus proximal tubule

R. London, B. Cohen, William B Guggino, G. Giebisch

Research output: Contribution to journalArticle

Abstract

In a previous study we presented evidence that chloride transport across the basolateral membrane in Necturus proximal tubule cells occurs predominantly via exchange for both Na+ and HCO3-. In this study the regulation of intracellular chloride was further examined in the doubly-perfused kidney preparation using conventional and chloride-sensitive microelectrodes. Application of hypertonic basolateral solutions containing 80 mm raffinose stimulated an efflux of chloride such that chloride activity remained unchanged at control levels. Membrane potential did not change in these experiments. Inhibition of Cl- exit across the basolateral cell membrane by removal of either HCO3- or Na+ from the perfusion solution resulted in a significant increase in intracellular chloride activity, aCli, when basolateral osmolarity was raised. Hypertonic basolateral solutions also produced a significant rise in aCli in the presence of SITS. This study provides further evidence that chloride is transported across the basolateral cell membrane in exchange for both Na+ and HCO3-. Since this exchange mechanism is activated in response to hypertonic solutions, these studies suggest a functional role for this exchanger in the regulation of aCli in the Necturus proximal tubule cell during volume changes.

Original languageEnglish (US)
Pages (from-to)253-258
Number of pages6
JournalJournal of Membrane Biology
Volume75
Issue number3
DOIs
StatePublished - Oct 1983
Externally publishedYes

Fingerprint

Necturus
Hypertonic Solutions
Chlorides
Perfusion
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Cell Membrane
Raffinose
Microelectrodes
Cell Size
Membrane Potentials
Osmolar Concentration
Kidney
Membranes

Keywords

  • basolateral membrane
  • chloride-bicarbonate exchange
  • hypertonic conditions
  • Necturus proximal tubule

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Regulation of intracellular chloride activity during perfusion with hypertonic solutions in the Necturus proximal tubule. / London, R.; Cohen, B.; Guggino, William B; Giebisch, G.

In: Journal of Membrane Biology, Vol. 75, No. 3, 10.1983, p. 253-258.

Research output: Contribution to journalArticle

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