Regulation of human T-Cell production of interleukin 2 by Leu 11 (CD16) positive large granular lymphocytes

Zahra Toossi, Michelle R. Paris, Scott F. Purvis, Jerrold J. Ellner

Research output: Contribution to journalArticle

Abstract

Peripheral blood large granular lymphocytes (LGL) expressing Leu 11 (CD16) antigen with potent natural killer cytotoxicity inhibited soluble antigen-induced T-cell production of interleukin 2 (IL-2). Depletion of Leu 11-reactive cells from T-cells doubled IL-2 activity (P <0.05). Leu 11 -enriched cells did not express high affinity IL-2 receptors nor did they deplete IL-2 activity from culture media. Upon addition in low ratios to Leu 11-depleted cells, Leu 11-enriched fractions increased antigen-induced IL-2 production three-fold (P <0.05), whereas at higher ratios IL-2 production was suppressed P <0.01. Additionally, adherent monocytes were increasingly accessory when added in graded numbers to Leu 11-depleted but not T-cell cultures. In the presence of small numbers (5%) of Leu 11-enriched cells, however, monocytes down-regulated IL-2 production of Leu 11-depleted cell cultures. Thus Leu 11-reactive lymphocytes have noncytotoxic functions and may play a major role in immunoregulation.

Original languageEnglish (US)
Pages (from-to)413-424
Number of pages12
JournalCellular Immunology
Volume118
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

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Interleukin-2
Lymphocytes
T-Lymphocytes
IgG Receptors
Monocytes
Cell Culture Techniques
Antigens
Interleukin-2 Receptors
Culture Media

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Regulation of human T-Cell production of interleukin 2 by Leu 11 (CD16) positive large granular lymphocytes. / Toossi, Zahra; Paris, Michelle R.; Purvis, Scott F.; Ellner, Jerrold J.

In: Cellular Immunology, Vol. 118, No. 2, 1989, p. 413-424.

Research output: Contribution to journalArticle

Toossi, Zahra ; Paris, Michelle R. ; Purvis, Scott F. ; Ellner, Jerrold J. / Regulation of human T-Cell production of interleukin 2 by Leu 11 (CD16) positive large granular lymphocytes. In: Cellular Immunology. 1989 ; Vol. 118, No. 2. pp. 413-424.
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