Regulation of human calcitonin gene transcription by cyclic AMP

Andrée de Bustros, Douglas W. Ball, Randolph Peters, Debra Compton, Barry D. Nelkin

Research output: Contribution to journalArticlepeer-review

Abstract

Transcription of the human calcitonin (CT) gene is markedly increased by cAMP in the TT line of medullary thyroid carcinoma. This response is conferred by 5′ flanking DNA sequences located between -132 and -252 relative to the transcription initiation site. Within this region are an upstream cyclic AMP response element (CRE), a downstream CRE flanked by two octamer motifs, and two adjacent C-rich AP2-like sequences. In transfection experiments in TT cells, the downstream CRE, combined with CT promoter sequences, generated 70% of the maximal cAMP response. The upstream CRE and the C-rich elements conferred 10 and 30% of this response, respectively. In gel mobility shift assays, specific TT cell proteins bound to each of these sequences. Therefore, the cAMP response of the CT gene is complex, requiring multiple elements acting in concert.

Original languageEnglish (US)
Pages (from-to)1157-1164
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume189
Issue number2
DOIs
StatePublished - Dec 15 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Regulation of human calcitonin gene transcription by cyclic AMP'. Together they form a unique fingerprint.

Cite this