Regulation of hematopoiesis IV: The role of interleukin-3 and bryostatin 1 in the growth of erythropoietic progenitors from normal and anemic W/W(v) mice

J. P. Leonard, W. S. May, J. N. Ihle, G. R. Pettit, S. J. Sharkis

Research output: Contribution to journalArticle

Abstract

We and others have established a role for T lymphocytes and their products in the regulation of erythropoiesis. Interleukin-3 (IL-3) is a multipotential lymphokine with burst-promoting activity that is produced by activated T lymphocytes. In the anemic, stem cell-defective W/W(v) mouse we have described the absence of a functionally active thymocyte population that in normal animals enhances erythroid progenitor growth and stem cell self-renewal. In studies reported here we find that W/W(v) mouse marrow responds to exogenous IL-3 by increased erythroid progenitor cell growth. The BFU-E and CFU-E from anemic donors are more sensitive to IL-3 than are those in +/+ marrow. We have recently observed a stimulatory effect of bryostatin 1 (a macrocyclic lactone derived from a marine invertebrate) on normal erythropoiesis in human bone marrow progenitor assays. To test the effects of this molecule on murine normal and anemic W/W(v) cells we grew these cells in the presence of increasing doses of bryostatin 1. Bryostatin mimics the stimulatory action of IL-3 on W/W(v) bone marrow. Polyclonal antibody directed against murine IL-3 blocks the stimulatory effect of bryostatin on erythropoiesis. Otherwise inactive thymocytes from W/W(v) mice in coculture with W/W(v) bone marrow showed stimulation of erythropoiesis in the presence of bryostatin. We believe that bryostatin may in part act by stimulating T lymphocytes to release physiologic concentrations of lymphokines.

Original languageEnglish (US)
Pages (from-to)1492-1496
Number of pages5
JournalBlood
Volume72
Issue number5
StatePublished - 1988

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Erythroid Precursor Cells
Bryostatins
Interleukin-3
Hematopoiesis
Interleukin-1
Erythropoiesis
Bone Marrow
T-cells
Growth
Bone
Lymphokines
Thymocytes
Stem cells
T-Lymphocytes
Cell growth
Lactones
Invertebrates
Coculture Techniques
Assays
Animals

ASJC Scopus subject areas

  • Hematology

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Regulation of hematopoiesis IV : The role of interleukin-3 and bryostatin 1 in the growth of erythropoietic progenitors from normal and anemic W/W(v) mice. / Leonard, J. P.; May, W. S.; Ihle, J. N.; Pettit, G. R.; Sharkis, S. J.

In: Blood, Vol. 72, No. 5, 1988, p. 1492-1496.

Research output: Contribution to journalArticle

Leonard, J. P. ; May, W. S. ; Ihle, J. N. ; Pettit, G. R. ; Sharkis, S. J. / Regulation of hematopoiesis IV : The role of interleukin-3 and bryostatin 1 in the growth of erythropoietic progenitors from normal and anemic W/W(v) mice. In: Blood. 1988 ; Vol. 72, No. 5. pp. 1492-1496.
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