Regulation of Heat Shock Transcription Factor 1 by Stress-induced SUMO-1 Modification

Yiling Hong, Richard Rogers, Michael J. Matunis, Christopher N. Mayhew, Michael Goodson, Ok Kyong Park-Sarge, Kevin D. Sarge

Research output: Contribution to journalArticle

Abstract

Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress HSF1 acquires DNA binding ability and localizes to nuclear stress granules, but the molecular mechanisms that mediate these events are not understood. We report that HSF1 undergoes stress-induced modification at lysine 298 by the small ubiquitin-related protein called SUMO-1. Antibodies against SUMO-1 supershift the HSF1 DNA-binding complex, and modification of HSF1 in a reconstituted SUMO-1 reaction system causes conversion of HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of lysine 298. Mutation of lysine 298 also results in a significant decrease in stress-induced transcriptional activity of HSF1 in vivo. This work implicates SUMO-1 modification as an important modulator of HSF1 function in response to stress.

Original languageEnglish (US)
Pages (from-to)40263-40267
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number43
DOIs
StatePublished - Oct 26 2001

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hong, Y., Rogers, R., Matunis, M. J., Mayhew, C. N., Goodson, M., Park-Sarge, O. K., & Sarge, K. D. (2001). Regulation of Heat Shock Transcription Factor 1 by Stress-induced SUMO-1 Modification. Journal of Biological Chemistry, 276(43), 40263-40267. https://doi.org/10.1074/jbc.M104714200