TY - JOUR
T1 - Regulation of gene expression by a metabolic enzyme
AU - Hall, David A.
AU - Zhu, Heng
AU - Zhu, Xiaowei
AU - Royce, Thomas
AU - Gerstein, Mark
AU - Snyder, Michael
PY - 2004/10/15
Y1 - 2004/10/15
N2 - Gene expression in eukaryotes is normally believed to be controlled by transcriptional regulators that activate genes encoding structural proteins and enzymes. To identify previously unrecognized DNA binding activities, a yeast proteome microarray was screened with DNA probes; Arg5,6, a well-characterized mitochondrial enzyme involved in arginine biosynthesis, was identified. Chromatin immunoprecipitation experiments revealed that Arg5,6 is associated with specific nuclear and mitochondrial loci in vivo, and Arg5,6 binds to specific fragments in vitro. Deletion of Arg5,6 causes altered transcript levels of both nuclear and mitochondrial target genes. These results indicate that metabolic enzymes can directly regulate eukaryotic gene expression.
AB - Gene expression in eukaryotes is normally believed to be controlled by transcriptional regulators that activate genes encoding structural proteins and enzymes. To identify previously unrecognized DNA binding activities, a yeast proteome microarray was screened with DNA probes; Arg5,6, a well-characterized mitochondrial enzyme involved in arginine biosynthesis, was identified. Chromatin immunoprecipitation experiments revealed that Arg5,6 is associated with specific nuclear and mitochondrial loci in vivo, and Arg5,6 binds to specific fragments in vitro. Deletion of Arg5,6 causes altered transcript levels of both nuclear and mitochondrial target genes. These results indicate that metabolic enzymes can directly regulate eukaryotic gene expression.
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U2 - 10.1126/science.1096773
DO - 10.1126/science.1096773
M3 - Article
C2 - 15486299
AN - SCOPUS:5644229499
SN - 0036-8075
VL - 306
SP - 482
EP - 484
JO - Science
JF - Science
IS - 5695
ER -