Regulation of gene expression by a metabolic enzyme

David A. Hall, Heng Zhu, Xiaowei Zhu, Thomas Royce, Mark Gerstein, Michael Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

Gene expression in eukaryotes is normally believed to be controlled by transcriptional regulators that activate genes encoding structural proteins and enzymes. To identify previously unrecognized DNA binding activities, a yeast proteome microarray was screened with DNA probes; Arg5,6, a well-characterized mitochondrial enzyme involved in arginine biosynthesis, was identified. Chromatin immunoprecipitation experiments revealed that Arg5,6 is associated with specific nuclear and mitochondrial loci in vivo, and Arg5,6 binds to specific fragments in vitro. Deletion of Arg5,6 causes altered transcript levels of both nuclear and mitochondrial target genes. These results indicate that metabolic enzymes can directly regulate eukaryotic gene expression.

Original languageEnglish (US)
Pages (from-to)482-484
Number of pages3
JournalScience
Volume306
Issue number5695
DOIs
StatePublished - Oct 15 2004

ASJC Scopus subject areas

  • General

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