Regulation of expression of branched-chain α-keto acid dehydrogenase subunits in permanent cell lines

Jeffrey M. Chinsky, Paul A. Costeas

Research output: Contribution to journalArticlepeer-review

Abstract

The rat hepatoma cell line H4IIEC3 has demonstrated a response to both insulin and glucocorticoids in its accumulation of BCKAD subunit RNAs. It is amenable to BCKAD promoter minigene transfection analyses, demonstrating positive (glucocorticoids) and negative (insulin) regulatory effects. These cells can therefore be used as a model to identify cis-acting sites responsible for regulation of BCKAD subunit promoter activity.

Original languageEnglish (US)
Pages (from-to)479-490
Number of pages12
JournalMethods in enzymology
Volume324
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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