TY - JOUR
T1 - Regulation of endothelial cell adherens junctions by a ras-dependent signal transduction pathway
AU - Hegland, Donald D.
AU - Sullivan, Daniel M.
AU - Rovira, Ilsa I.
AU - Li, Arthur
AU - Kovesdi, Imre
AU - Bruder, Joseph T.
AU - Finkel, Toren
PY - 1999/7/5
Y1 - 1999/7/5
N2 - Adherens junctions, consisting of transmembrane cadherin molecules and their associated cytoplasmic α-, β-, and γ-catenin proteins, are thought to be critical for the development of stable cell adhesion and subsequent 3-dimensional tissue organization. In human endothelial cells there is a marked induction of γ-catenin levels when cells reach confluence. We demonstrate that expression of a dominant negative ras gene product (N17ras) via adenoviral mediated gene transfer inhibits the confluent-dependent rise in γ-catenin mRNA and protein levels. Consistent with its effects on overall γ-catenin levels, expression of N17ras also reduces the amount of γ-catenin associated with the adherens junction. Finally, although expression of N17ras under normal culture conditions produces no clear morphological phenotype, endothelial cells expressing a dominant negative ras gene product fail to form 3-dimensional, vascular-like structures when plated on reconstituted extracellular matrix.
AB - Adherens junctions, consisting of transmembrane cadherin molecules and their associated cytoplasmic α-, β-, and γ-catenin proteins, are thought to be critical for the development of stable cell adhesion and subsequent 3-dimensional tissue organization. In human endothelial cells there is a marked induction of γ-catenin levels when cells reach confluence. We demonstrate that expression of a dominant negative ras gene product (N17ras) via adenoviral mediated gene transfer inhibits the confluent-dependent rise in γ-catenin mRNA and protein levels. Consistent with its effects on overall γ-catenin levels, expression of N17ras also reduces the amount of γ-catenin associated with the adherens junction. Finally, although expression of N17ras under normal culture conditions produces no clear morphological phenotype, endothelial cells expressing a dominant negative ras gene product fail to form 3-dimensional, vascular-like structures when plated on reconstituted extracellular matrix.
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U2 - 10.1006/bbrc.1999.0919
DO - 10.1006/bbrc.1999.0919
M3 - Article
C2 - 10403777
AN - SCOPUS:0033526778
VL - 260
SP - 371
EP - 376
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -