Regulation of Endogenous SH2 Domain-Containing Inositol 5-Phosphatase (SHIP2) in 3T3-L1 and Human Preadipocytes

Annemarie Gagnon, Yulia Artemenko, Thet Crapper, Alexander Sorisky

Research output: Contribution to journalArticle

Abstract

The role of the inositol lipid 5-phosphatase (SHIP2) in preadipocyte signaling is not known. Although overexpression of SHIP2 inhibited proliferation and 3H-thymidine incorporation in 3T3-L1 preadipocytes, there was no effect on insulin-induced adipogenesis. Insulin promoted SHIP2 tyrosine phosphorylation in differentiated 3T3-L1 adipocytes, but did not do so in preadipocytes. The absence of SHIP2 tyrosine phosphorylation suggests a potential explanation for the isolated rise in PI(3,4,5)P3, without any changes in PI(3,4)P2, previously observed following insulin treatment of these cells. Lack of SHIP2 tyrosine phosphorylation by insulin was also observed in primary cultures of human abdominal subcutaneous preadipocytes. These cells also produced PI(3,4,5)P3, but not PI(3,4)P2, in response to insulin. Comparison of insulin vs. PDGF treatment on SHIP2 tyrosine phosphorylation in 3T3-L1 and human preadipocytes revealed that only PDGF, which stimulates the accumulation of PI(3,4,5)P3 as well as PI(3,4)P2, was active in this regard, and only PDGF promoted the association of 52 kDa form of Shc with SHIP2. Nevertheless, both insulin and PDGF were equally effective in translocating SHIP2 to the plasma membrane in 3T3-L1 preadipocytes. Lack of SHIP2 tyrosine phosphorylation may account for the insulin-specific inositol phospholipid pattern of accumulation in preadipocytes.

Original languageEnglish (US)
Pages (from-to)243-250
Number of pages8
JournalJournal of Cellular Physiology
Volume197
Issue number2
DOIs
StatePublished - Nov 2003
Externally publishedYes

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src Homology Domains
Inositol
Phosphoric Monoester Hydrolases
Phosphorylation
Insulin
Tyrosine
Adipogenesis
Inositol Polyphosphate 5-Phosphatases
Cell membranes
Phosphatidylinositols
Adipocytes
Thymidine
Cells
Cell Membrane
Association reactions
Lipids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Regulation of Endogenous SH2 Domain-Containing Inositol 5-Phosphatase (SHIP2) in 3T3-L1 and Human Preadipocytes. / Gagnon, Annemarie; Artemenko, Yulia; Crapper, Thet; Sorisky, Alexander.

In: Journal of Cellular Physiology, Vol. 197, No. 2, 11.2003, p. 243-250.

Research output: Contribution to journalArticle

Gagnon, Annemarie ; Artemenko, Yulia ; Crapper, Thet ; Sorisky, Alexander. / Regulation of Endogenous SH2 Domain-Containing Inositol 5-Phosphatase (SHIP2) in 3T3-L1 and Human Preadipocytes. In: Journal of Cellular Physiology. 2003 ; Vol. 197, No. 2. pp. 243-250.
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