Regulation of E2A gene expression in B-lymphocyte development

Yuan Zhuang, Annette Jackson, Lihua Pan, Kang Shen, Meifang Dai

Research output: Contribution to journalArticlepeer-review

Abstract

Biochemical and genetic studies have demonstrated that transcription factors encoded by the E2A gene are essential in regulating B lineage specific gene expression and B lineage commitment. However, the mechanism by which E2A regulates B lineage commitment is not known. It has been reported that E2A controls B lineage commitment in a dosage dependent manner. To further investigate this gene dosage effect, we analyzed E2A expression during normal B cell development in mice carrying a functional E2AGFP knockin allele. Mice carrying this fusion allele were examined for E2A gene expression during bone marrow B cell development. A dramatic upregulation of E2A is observed concomitant with the initiation of immunoglobulin heavy chain D-J rearrangement and the induction of Early B cell Factor (EBF) gene expression. We also show that this E2A upregulation does not occur in the absence of the EBF gene. These results indicate that E2A upregulation is a critical step in regulating B-lineage commitment. It further suggests that E2A gene dosage may be determined by a cross regulation between E2A and EBF during B lineage commitment.

Original languageEnglish (US)
Pages (from-to)1165-1177
Number of pages13
JournalMolecular Immunology
Volume40
Issue number16
DOIs
StatePublished - Mar 2004

Keywords

  • BHLH
  • EBF
  • GFP
  • Immunoglobulin genes
  • Knockin

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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