Regulation of Cell Adhesion and Collective Cell Migration by Hindsight and Its Human Homolog RREB1

Mariana Melani, Kaylene J. Simpson, Joan S. Brugge, Denise Montell

Research output: Contribution to journalArticle

Abstract

Cell movements represent a major driving force in embryonic development, tissue repair, and tumor metastasis [1]. The migration of single cells has been well studied, predominantly in cell culture [2, 3]; however, in vivo, a greater variety of modes of cell movement occur, including the movements of cells in clusters, strands, sheets, and tubes, also known as collective cell migrations [4, 5]. In spite of the relevance of these types of movements in both normal and pathological conditions, the molecular mechanisms that control them remain predominantly unknown. Epithelial follicle cells of the Drosophila ovary undergo several dynamic morphological changes, providing a genetically tractable model [6]. We found that anterior follicle cells, including border cells, mutant for the gene hindsight (hnt) accumulated excess cell-cell adhesion molecules and failed to undergo their normal collective movements. In addition, HNT affected border cell cluster cohesion and motility via effects on the JNK and STAT pathways, respectively. Interestingly, reduction of expression of the mammalian homolog of HNT, RREB1, by siRNA inhibited collective cell migration in a scratch-wound healing assay of MCF10A mammary epithelial cells, suppressed surface activity, retarded cell spreading after plating, and led to the formation of immobile, tightly adherent cell colonies. We propose that HNT and RREB1 are essential to reduce cell-cell adhesion when epithelial cells within an interconnected group undergo dynamic changes in cell shape.

Original languageEnglish (US)
Pages (from-to)532-537
Number of pages6
JournalCurrent Biology
Volume18
Issue number7
DOIs
StatePublished - Apr 8 2008

Fingerprint

Cell adhesion
cell movement
cell adhesion
Cell Adhesion
Cell Movement
Cells
Cell Adhesion Molecules
cells
Cell culture
Plating
Epithelial Cells
Small Interfering RNA
Tumors
Assays
Repair
Genes
Tissue
tissue repair
Cell Shape
MAP Kinase Signaling System

Keywords

  • CELLBIO
  • DEVBIO
  • SIGNALING

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Regulation of Cell Adhesion and Collective Cell Migration by Hindsight and Its Human Homolog RREB1. / Melani, Mariana; Simpson, Kaylene J.; Brugge, Joan S.; Montell, Denise.

In: Current Biology, Vol. 18, No. 7, 08.04.2008, p. 532-537.

Research output: Contribution to journalArticle

Melani, Mariana ; Simpson, Kaylene J. ; Brugge, Joan S. ; Montell, Denise. / Regulation of Cell Adhesion and Collective Cell Migration by Hindsight and Its Human Homolog RREB1. In: Current Biology. 2008 ; Vol. 18, No. 7. pp. 532-537.
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