Regulation of aquaporin-2 by RNA interference

Hyun Jun Jung, Eui Jung Park, Hyo Jung Choi, Tae Hwan Kwon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs (miRNAs) are short non-coding RNAs that interact with 3′-untranslated regions of mRNAs. miRNAs modulate gene expression by regulating mRNA translation and provide novel insights into the complex regulation of protein expression and function. In this chapter, we describe the general features of RNA interference, identification of miRNAs, and interaction of miRNAs with their target genes. In particular, we have shown that several miRNAs are responsive to arginine vasopressin or aldosterone stimulation in mouse cortical collecting duct mpkCCD cells. Moreover, we identified both miR-32 and miR-137 as AQP2-targeting miRNAs using in silico analysis and also identified several target genes of miR-32 and miR-137. As the target sequences of miR-32 and miR-137 are commonly found in mRNAs of vasopressin-regulated genes, further studies regarding the interaction of miRNAs with their target genes are required to obtain comprehensive understanding of miRNA-regulated AQP2 expression in kidney collecting duct cells.

Original languageEnglish (US)
Title of host publicationVitamins and Hormones
EditorsGerald Litwack
PublisherAcademic Press Inc.
Pages119-145
Number of pages27
ISBN (Print)9780128208090
DOIs
StatePublished - 2020

Publication series

NameVitamins and Hormones
Volume112
ISSN (Print)0083-6729

Keywords

  • Aquaporin-2
  • Collecting duct
  • MicroRNA
  • Post-transcriptional regulation
  • RNA interference
  • Small interfering RNA
  • Vasopressin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology

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