TY - JOUR
T1 - Regulation of α2(I) collagen expression in stellate cells by retinoic acid and retinoid X receptors through interactions with their cofactors
AU - Wang, Lan
AU - Tankersley, Lynda Rennie
AU - Tang, Mei
AU - Potter, James J.
AU - Mezey, Esteban
N1 - Funding Information:
This work is supported by Grants AA00323 and AA00626 from the United States Public Health Services. M.T. was postdoctoral fellow on National Research Service Award 2 T32 AA07467 from the National Institute on Alcohol Abuse and Alcoholism, NIH. We thank Drs. Ronald M. Evans, Pierre Chambon, Michael R. Stallcup, and Ming-Jer Tsai, for their generous gifts of expression vectors.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Retinoic acid (RA) suppresses α2(I) collagen expression in hepatic stellate cells through the binding of retinoic acid receptor β (RARβ) and retinoid X receptor α (RXRα) to RA response elements (RAREs) in the α2(I) collagen promoter. This study determined the influence of coactivators and corepressors to RARβ and RXRα on the regulation of the α2(I) collagen promoter. The coactivators, steroid receptor coactivator-1 (SRC-1) and growth hormone receptor interacting protein-1 (GRIP-1), enhanced, while the nuclear receptor corepressor (N-CoR) abolished the inhibitory effect of RARβ and RXRα on the promoter activity. In the presence of RA, the coactivators SRC-1 and GRIP-1 formed complexes with RARβ and RXRα which are bound to an oligonucleotide specifying a RARE site in the promoter. In conclusion, this study shows that in the presence of retinoic acid, the coactivators SRC-1 and GRIP-1 augment, while the corepressor N-CoR abolishes, the suppressive effects of RARβ and RXRα on α2(I) collagen promoter activity.
AB - Retinoic acid (RA) suppresses α2(I) collagen expression in hepatic stellate cells through the binding of retinoic acid receptor β (RARβ) and retinoid X receptor α (RXRα) to RA response elements (RAREs) in the α2(I) collagen promoter. This study determined the influence of coactivators and corepressors to RARβ and RXRα on the regulation of the α2(I) collagen promoter. The coactivators, steroid receptor coactivator-1 (SRC-1) and growth hormone receptor interacting protein-1 (GRIP-1), enhanced, while the nuclear receptor corepressor (N-CoR) abolished the inhibitory effect of RARβ and RXRα on the promoter activity. In the presence of RA, the coactivators SRC-1 and GRIP-1 formed complexes with RARβ and RXRα which are bound to an oligonucleotide specifying a RARE site in the promoter. In conclusion, this study shows that in the presence of retinoic acid, the coactivators SRC-1 and GRIP-1 augment, while the corepressor N-CoR abolishes, the suppressive effects of RARβ and RXRα on α2(I) collagen promoter activity.
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U2 - 10.1016/j.abb.2004.05.004
DO - 10.1016/j.abb.2004.05.004
M3 - Article
C2 - 15234273
AN - SCOPUS:3042636432
SN - 0003-9861
VL - 428
SP - 92
EP - 98
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -