Regulation and function of Gα protein subunits in Dictyostelium

A. Kumagai, M. Pupillo, R. Gundersen, R. Miake-Lye, Peter N Devreotes, R. A. Firtel

Research output: Contribution to journalArticle

Abstract

We have examined the developmental regulation and function of two Gα protein subunits, Gα1 and Gα2, from Dictyostelium. Gα1 is expressed in vegetative cells through aggregate stages while Gα2 is inducible by cAMP pulses and preferentially expressed in aggregation. Our results suggest that Gα2 encodes the Gα protein subunit associated with the cAMP receptor and mediates all known receptor-activated intracellular signal transduction processes, including chemotaxis and gene regulation. Gα1 appears to function in both the cell cycle and development. Overexpression of Gα1 results in large, multinucleated cells that develop abnormally. The central role that these Gα proteins play in signal transduction processes and in controlling Dictyostelium development is discussed.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalCell
Volume57
Issue number2
DOIs
StatePublished - Apr 21 1989

Fingerprint

Dictyostelium
Protein Subunits
GTP-Binding Proteins
Signal transduction
Signal Transduction
Cyclic AMP Receptors
Chemotaxis
Gene expression
Cell Cycle
Agglomeration
Cells
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Kumagai, A., Pupillo, M., Gundersen, R., Miake-Lye, R., Devreotes, P. N., & Firtel, R. A. (1989). Regulation and function of Gα protein subunits in Dictyostelium. Cell, 57(2), 265-275. https://doi.org/10.1016/0092-8674(89)90964-1

Regulation and function of Gα protein subunits in Dictyostelium. / Kumagai, A.; Pupillo, M.; Gundersen, R.; Miake-Lye, R.; Devreotes, Peter N; Firtel, R. A.

In: Cell, Vol. 57, No. 2, 21.04.1989, p. 265-275.

Research output: Contribution to journalArticle

Kumagai, A, Pupillo, M, Gundersen, R, Miake-Lye, R, Devreotes, PN & Firtel, RA 1989, 'Regulation and function of Gα protein subunits in Dictyostelium', Cell, vol. 57, no. 2, pp. 265-275. https://doi.org/10.1016/0092-8674(89)90964-1
Kumagai A, Pupillo M, Gundersen R, Miake-Lye R, Devreotes PN, Firtel RA. Regulation and function of Gα protein subunits in Dictyostelium. Cell. 1989 Apr 21;57(2):265-275. https://doi.org/10.1016/0092-8674(89)90964-1
Kumagai, A. ; Pupillo, M. ; Gundersen, R. ; Miake-Lye, R. ; Devreotes, Peter N ; Firtel, R. A. / Regulation and function of Gα protein subunits in Dictyostelium. In: Cell. 1989 ; Vol. 57, No. 2. pp. 265-275.
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