Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis

Hana Bruchova, Donghoon Yoon, Archana M. Agarwal, Joshua Mendell, Josef T. Prchal

Research output: Contribution to journalArticle

Abstract

Objective: Polycythemia vera (PV) is a myeloproliferative disorder, arising from the acquired mutation(s) of a hematopoietic stem cell. The JAK2 V617F somatic mutation is found in most PV patients; however, it is not the disease-initiating mutation. Because microRNAs (miRNAs) play a regulatory role in hematopoiesis, we studied miRNA expressions in PV and normal erythropoiesis. Methods: Peripheral blood mononuclear cells were cultured in a three-phase liquid system resulting in synchronized expansion of erythroid progenitors. Using gene-expression profiling by CombiMatrix MicroRNArray, we searched for PV-specific changes at days 1, 14, and 21. Twelve miRNA candidates were then reevaluated by quantitative real-time polymerase chain reaction in a larger number of samples obtained from progenitors at the same stage of differentiation. Results: A significant difference in miR-150 expression was found in PV. In normal erythropoiesis, three expression patterns of miRNAs were observed: progressive downregulation of miR-150, miR-155, miR-221, miR-222; upregulation of miR-451, miR-16 at late stages of erythropoiesis; and biphasic regulation of miR-339, miR-378. The miR-451 appears to be erythroid-specific. Conclusions: We identified the miRNAs with regulated expression in erythropoiesis; one appeared to be PV-specific. Their miRNA expression levels define early, intermediate, and late stages of erythroid differentiation. The validity of our findings was confirmed in nonexpanded peripheral blood cells.

Original languageEnglish (US)
Pages (from-to)1657-1667
Number of pages11
JournalExperimental Hematology
Volume35
Issue number11
DOIs
StatePublished - Nov 2007

Fingerprint

Polycythemia Vera
Erythropoiesis
MicroRNAs
Mutation
Blood Cells
Myeloproliferative Disorders
Hematopoiesis
Gene Expression Profiling
Hematopoietic Stem Cells
Real-Time Polymerase Chain Reaction
Up-Regulation
Down-Regulation

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Bruchova, H., Yoon, D., Agarwal, A. M., Mendell, J., & Prchal, J. T. (2007). Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis. Experimental Hematology, 35(11), 1657-1667. https://doi.org/10.1016/j.exphem.2007.08.021

Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis. / Bruchova, Hana; Yoon, Donghoon; Agarwal, Archana M.; Mendell, Joshua; Prchal, Josef T.

In: Experimental Hematology, Vol. 35, No. 11, 11.2007, p. 1657-1667.

Research output: Contribution to journalArticle

Bruchova, H, Yoon, D, Agarwal, AM, Mendell, J & Prchal, JT 2007, 'Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis', Experimental Hematology, vol. 35, no. 11, pp. 1657-1667. https://doi.org/10.1016/j.exphem.2007.08.021
Bruchova, Hana ; Yoon, Donghoon ; Agarwal, Archana M. ; Mendell, Joshua ; Prchal, Josef T. / Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis. In: Experimental Hematology. 2007 ; Vol. 35, No. 11. pp. 1657-1667.
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