Regional metabolic status of the E-18 rat fetal brain following transient hypoxia/ischemia

Svetlana Pundik, Shenandoah Robinson, W. David Lust, Jennifer Zechel, Marek Buczek, Warren R. Selman

Research output: Contribution to journalArticle

Abstract

Increasing evidence indicates that fetal metabolic stress may result in a variety of post-natal perturbations during brain development. The goal of the study was to determine the duration of hypoxia/ischemia that would elicit a demonstrable regional depression of metabolism in the fetal brain and further to examine several end-points to determine if the metabolic stress affects the developing brain. The uterine artery and uterine branch of the ovarian artery were occluded with aneurysm clamps for a period of 45 min, the clips removed and the metabolites in five regions of the perinatal brain were measured at 0, 2 and 6 h of reflow. Regional P-creatine, ATP and glucose levels were significantly depleted at the end of the 45 min occlusion. The levels of glycogen and glutamate at the end of the occlusion indicated a decreasing trend which was not significant. The concentration of citrate remained essentially unchanged at the end of the occlusion. To ensure that the insult was not lethal to the tissue, the recovery of the metabolites was examined at 2 and 6 h of reflow and generally the concentrations of the high-energy phosphates and glucose were normal or near-normal by 6 h of reperfusion in the five regions of the brain examined. The changes in the metabolites indicate that 45 min of hypoxia/ischemia is an appropriate model for studying neonatal development after fetal metabolic stress.

Original languageEnglish (US)
Pages (from-to)309-317
Number of pages9
JournalMetabolic Brain Disease
Volume21
Issue number4
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Keywords

  • Fetal metabolic Stress
  • Neonatal brain development

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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