TY - JOUR
T1 - Regional function-structure relationships in lungs of an elastase murine model of emphysema
AU - Ishii, Masaru
AU - Emami, Kiarash
AU - Xin, Yi
AU - Barulic, Amy
AU - Kotzer, Charles J.
AU - Logan, Gregory A.
AU - Chia, Elaine
AU - MacDuffie-Woodburn, John P.
AU - Zhu, Jianliang
AU - Pickup, Stephen
AU - Kuzma, Nicholas
AU - Kadlecek, Stephen
AU - Podolin, Patricia L.
AU - Rizi, Rahim R.
PY - 2012/1
Y1 - 2012/1
N2 - Changes in lung function and structure were studied using hyperpolarized 3He MRI in an elastaseinduced murine model of emphysema. The combined analysis of the apparent diffusion coefficient (ADC) and fractional ventilation (R) were used to distinguish emphysematous changes and also to develop a model for classifying sections of the lung into diseased and normal. Twelve healthy male BALB/c mice (26 × 2 g) were randomized into healthy and elastase-induced mice and studied ̃8-11 wk after model induction. ADC and R were measured at a submillimeter planar resolution. Chord length (Lx) data were analyzed from histology samples from the corresponding imaged slices. Logistic regression was applied to estimate the probability that an imaged pixel came from a diseased animal, and bootstrap methods (1,000 samples) were used to compare the regression results for the morphological and imaging results. Multivariate ANOVA (MANOVA) was used to analyze transformed ADC (ADCBC), and R (RBC) data and also to control for the experiment-wide error rate. MANOVA and ANOVA showed that elastase induced a statistically measureable change in the average transformed Lx and ADCBC but not in the average RBC. Marginal mean analysis demonstrated that ADCBC was on average 0.19 [95% confidence interval (CI): 0.16, 0.22] higher in the emphysema group, whereas RBC was on average 0.05 (95% CI: 0.04, 0.06) lower. Logistic regression supported the hypothesis that ADCBC and RBC, together, were better at differentiating normal from diseased tissue than either measurement alone. The odds ratios for ADCBC and RBC were 7.73 (95% CI: 5.23, 11.42) and 9.14 × 10 -5 (95% CI: 3.33 × 10 -5, 25.06 × 10 -5), respectively. Using a 50% probability cutoff, this model classified 70.6% of pixels correctly. The sensitivity and specificity of this model at the 50% cutoff were 74.9% and 65.2%, respectively. The area under the receiver operating characteristic curve was 0.76 (95% CI: 0.74, 0.78). The regression model presented can be used to map MRI data to disease probability maps. These probability maps present a future possibility of using both measurements in a more clinically feasible method of diagnosing this disease.
AB - Changes in lung function and structure were studied using hyperpolarized 3He MRI in an elastaseinduced murine model of emphysema. The combined analysis of the apparent diffusion coefficient (ADC) and fractional ventilation (R) were used to distinguish emphysematous changes and also to develop a model for classifying sections of the lung into diseased and normal. Twelve healthy male BALB/c mice (26 × 2 g) were randomized into healthy and elastase-induced mice and studied ̃8-11 wk after model induction. ADC and R were measured at a submillimeter planar resolution. Chord length (Lx) data were analyzed from histology samples from the corresponding imaged slices. Logistic regression was applied to estimate the probability that an imaged pixel came from a diseased animal, and bootstrap methods (1,000 samples) were used to compare the regression results for the morphological and imaging results. Multivariate ANOVA (MANOVA) was used to analyze transformed ADC (ADCBC), and R (RBC) data and also to control for the experiment-wide error rate. MANOVA and ANOVA showed that elastase induced a statistically measureable change in the average transformed Lx and ADCBC but not in the average RBC. Marginal mean analysis demonstrated that ADCBC was on average 0.19 [95% confidence interval (CI): 0.16, 0.22] higher in the emphysema group, whereas RBC was on average 0.05 (95% CI: 0.04, 0.06) lower. Logistic regression supported the hypothesis that ADCBC and RBC, together, were better at differentiating normal from diseased tissue than either measurement alone. The odds ratios for ADCBC and RBC were 7.73 (95% CI: 5.23, 11.42) and 9.14 × 10 -5 (95% CI: 3.33 × 10 -5, 25.06 × 10 -5), respectively. Using a 50% probability cutoff, this model classified 70.6% of pixels correctly. The sensitivity and specificity of this model at the 50% cutoff were 74.9% and 65.2%, respectively. The area under the receiver operating characteristic curve was 0.76 (95% CI: 0.74, 0.78). The regression model presented can be used to map MRI data to disease probability maps. These probability maps present a future possibility of using both measurements in a more clinically feasible method of diagnosing this disease.
KW - Apparent diffusion coefficient
KW - Hyperpolarized gas magnetic resonance imaging
KW - Lung physiology
KW - Regional fractional ventilation
KW - Relationship of lung function and structure
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U2 - 10.1152/japplphysiol.01181.2010
DO - 10.1152/japplphysiol.01181.2010
M3 - Article
C2 - 21940853
AN - SCOPUS:84855415217
SN - 8750-7587
VL - 112
SP - 135
EP - 148
JO - Journal of applied physiology
JF - Journal of applied physiology
IS - 1
ER -