Regenerate healing outcomes in unilateral mandibular distraction osteogenesis using quantitative histomorphometry

Daniel A. Schwarz, Krikor G. Arman, Mehreen S. Kakwan, Ameen Jamali, Ayman A. Elmeligy, Steven R. Buchman

Research output: Contribution to journalArticle

Abstract

Background: The authors' goal was to ascertain regenerate bone-healing metrics using quantitative histomorphometry at a single consolidation period. Methods: Rats underwent either mandibular distraction osteogenesis (n = 7) or partially reduced fractures (n = 7); their contralateral mandibles were used as controls (n = 11). External fixators were secured and unilateral osteotomies performed, followed by either mandibular distraction osteogenesis (4 days' latency, then 0.3 mm every 12 hours for 8 days; 5.1 mm) or partially reduced fractures (fixed immediately postoperatively; 2.1 mm); both groups underwent 4 weeks of consolidation. After tissue processing, bone volume/tissue volume ratio, osteoid volume/tissue volume ratio, and osteocyte count per high-power field were analyzed by means of quantitative histomorphometry. Results: Contralateral mandibles had statistically greater bone olume/tissue volume ratio and osteocyte count per high-power field compared with both mandibular distraction osteogenesis and partially reduced fractures by almost 50 percent, whereas osteoid volume/tissue volume ratio was statistically greater in both mandibular distraction osteogenesis specimens and partially reduced fractures compared with contralateral mandibles. No statistical difference in bone volume/tissue volume ratio, osteoid volume/tissue volume ratio, or osteocyte count per high-power field was found between mandibular distraction osteogenesis specimens and partially reduced fractures. Conclusions: The authors' findings demonstrate significantly decreased bone quantity and maturity in mandibular distraction osteogenesis specimens and partially reduced fractures compared with contralateral mandibles using the clinically analogous protocols. If these results are extrapolated clinically, treatment strategies may require modification to ensure reliable, predictable, and improved outcomes.

Original languageEnglish (US)
Pages (from-to)795-805
Number of pages11
JournalPlastic and Reconstructive Surgery
Volume126
Issue number3
DOIs
StatePublished - Sep 2010
Externally publishedYes

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Distraction Osteogenesis
Mandible
Osteocytes
Bone and Bones
External Fixators
Osteotomy

ASJC Scopus subject areas

  • Surgery

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Regenerate healing outcomes in unilateral mandibular distraction osteogenesis using quantitative histomorphometry. / Schwarz, Daniel A.; Arman, Krikor G.; Kakwan, Mehreen S.; Jamali, Ameen; Elmeligy, Ayman A.; Buchman, Steven R.

In: Plastic and Reconstructive Surgery, Vol. 126, No. 3, 09.2010, p. 795-805.

Research output: Contribution to journalArticle

Schwarz, Daniel A. ; Arman, Krikor G. ; Kakwan, Mehreen S. ; Jamali, Ameen ; Elmeligy, Ayman A. ; Buchman, Steven R. / Regenerate healing outcomes in unilateral mandibular distraction osteogenesis using quantitative histomorphometry. In: Plastic and Reconstructive Surgery. 2010 ; Vol. 126, No. 3. pp. 795-805.
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