Redundant DNA of Neurospora crassa

Robert R. Brooks; P. C. Huang

doi:10.1007/BF00485964

Redundant DNA of Neurospora crassa

Robert R. Brooks, P. C. Huang

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Approximately 20% of the DNA of Neurospora crassa consists of redundant sequences. This is calculated from the reassociation rate of fragmented, denatured DNA as measured by hydroxyapatite column chromatography. The redundant DNA has a complexity of 10⁵ base pairs and a repetition frequency of up to 60 copies per genome. Its buoyant density in CsCl is 1.720 g/ml and its hypochromicity 20-24%. Base composition determination shows 54% GC content like Neurospora nuclear DNA. DNA-RNA hybridization studies indicate that rRNA and tRNA cistrons make up 2.3 and 1.2%, respectively, of the redundant fraction. Pulse-labeled RNA is shown to hybridize with both redundant and unique DNA fractions, suggesting that both fractions are transcribed.

Original language	English (US)
Pages (from-to)	41-49
Number of pages	9
Journal	Biochemical Genetics
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/BF00485964
State	Published - Feb 1972
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics

Access to Document

10.1007/BF00485964

Cite this

@article{130d8f3d7747494ba4ad52bdd12c541c,

title = "Redundant DNA of Neurospora crassa",

abstract = "Approximately 20% of the DNA of Neurospora crassa consists of redundant sequences. This is calculated from the reassociation rate of fragmented, denatured DNA as measured by hydroxyapatite column chromatography. The redundant DNA has a complexity of 105 base pairs and a repetition frequency of up to 60 copies per genome. Its buoyant density in CsCl is 1.720 g/ml and its hypochromicity 20-24%. Base composition determination shows 54% GC content like Neurospora nuclear DNA. DNA-RNA hybridization studies indicate that rRNA and tRNA cistrons make up 2.3 and 1.2%, respectively, of the redundant fraction. Pulse-labeled RNA is shown to hybridize with both redundant and unique DNA fractions, suggesting that both fractions are transcribed.",

author = "Brooks, {Robert R.} and Huang, {P. C.}",

year = "1972",

month = feb,

doi = "10.1007/BF00485964",

language = "English (US)",

volume = "6",

pages = "41--49",

journal = "Biochemical Genetics",

issn = "0006-2928",

publisher = "Springer New York",

number = "1",

}

TY - JOUR

T1 - Redundant DNA of Neurospora crassa

AU - Brooks, Robert R.

AU - Huang, P. C.

PY - 1972/2

Y1 - 1972/2

N2 - Approximately 20% of the DNA of Neurospora crassa consists of redundant sequences. This is calculated from the reassociation rate of fragmented, denatured DNA as measured by hydroxyapatite column chromatography. The redundant DNA has a complexity of 105 base pairs and a repetition frequency of up to 60 copies per genome. Its buoyant density in CsCl is 1.720 g/ml and its hypochromicity 20-24%. Base composition determination shows 54% GC content like Neurospora nuclear DNA. DNA-RNA hybridization studies indicate that rRNA and tRNA cistrons make up 2.3 and 1.2%, respectively, of the redundant fraction. Pulse-labeled RNA is shown to hybridize with both redundant and unique DNA fractions, suggesting that both fractions are transcribed.

AB - Approximately 20% of the DNA of Neurospora crassa consists of redundant sequences. This is calculated from the reassociation rate of fragmented, denatured DNA as measured by hydroxyapatite column chromatography. The redundant DNA has a complexity of 105 base pairs and a repetition frequency of up to 60 copies per genome. Its buoyant density in CsCl is 1.720 g/ml and its hypochromicity 20-24%. Base composition determination shows 54% GC content like Neurospora nuclear DNA. DNA-RNA hybridization studies indicate that rRNA and tRNA cistrons make up 2.3 and 1.2%, respectively, of the redundant fraction. Pulse-labeled RNA is shown to hybridize with both redundant and unique DNA fractions, suggesting that both fractions are transcribed.

UR - http://www.scopus.com/inward/record.url?scp=0015295014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015295014&partnerID=8YFLogxK

U2 - 10.1007/BF00485964

DO - 10.1007/BF00485964

M3 - Article

C2 - 4269805

AN - SCOPUS:0015295014

SN - 0006-2928

VL - 6

SP - 41

EP - 49

JO - Biochemical Genetics

JF - Biochemical Genetics

IS - 1

ER -

Redundant DNA of Neurospora crassa

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this