TY - JOUR
T1 - Reduction of motion artifacts during in vivo two-photon imaging of brain through heartbeat triggered scanning
AU - Paukert, Martin
AU - Bergles, Dwight E.
PY - 2012/7
Y1 - 2012/7
N2 - Two-photon imaging of fluorescence in brain enables analysis of the structure and dynamic activity of neurons and glial cells in living animals. However, vital functions such as beating of the heart cause pulsations in brain tissue, leading to image distortion and loss of resolution. We find that synchronizing imaging scans to the cardiac cycle reduces motion artifacts, significantly improving the resolution of cellular structures. By interlacing multiple heartbeat triggered imaging scans, it was possible to image large brain volumes with negligible distortion. This approach can be readily incorporated into conventional microscopes to achieve substantial reductions in motion artifacts during two-photon imaging.
AB - Two-photon imaging of fluorescence in brain enables analysis of the structure and dynamic activity of neurons and glial cells in living animals. However, vital functions such as beating of the heart cause pulsations in brain tissue, leading to image distortion and loss of resolution. We find that synchronizing imaging scans to the cardiac cycle reduces motion artifacts, significantly improving the resolution of cellular structures. By interlacing multiple heartbeat triggered imaging scans, it was possible to image large brain volumes with negligible distortion. This approach can be readily incorporated into conventional microscopes to achieve substantial reductions in motion artifacts during two-photon imaging.
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U2 - 10.1113/jphysiol.2012.228114
DO - 10.1113/jphysiol.2012.228114
M3 - Article
C2 - 22508962
AN - SCOPUS:84862980324
SN - 0022-3751
VL - 590
SP - 2955
EP - 2963
JO - Journal of Physiology
JF - Journal of Physiology
IS - 13
ER -