Reduction of endogenous angiogenesis inhibitors in bruch's membrane of the submacular region in eyes with age-related macular degeneration

Imran A. Bhutto, Koichi Uno, Carol Merges, Lei Zhang, D. Scott McLeod, Gerard Anthony Lutty

Research output: Contribution to journalArticle

Abstract

Objectives: To determine the relative levels of 3 potent inhibitors of angiogenesis (endostatin, pigment epithelium-derived factor, and thrombospondin 1) in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex in the submacular region in aged control eyes and eyes with age-related macular degeneration (AMD). Methods: Immunohistochemical analysis with antibodies against endostatin, pigment epithelium-derived factor, and thrombospondin 1 was performed on the macular region of aged control donor eyes (n=8; mean age, 79.8 years) and eyes with AMD (n=12; mean age, 83.9 years). Three independent masked observers scored the reaction product (scored from 0-7). Mean scores from the control eyes and the eyes with AMD were analyzed using 1-way analysis of variance and unpaired t test. Results: In control eyes, strong immunoreactivity of all 3 inhibitors was observed in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex. Immunoreactivity for endostatin, pigment epithelium-derived factor, and thrombospondin 1 in Bruch's membrane was significantly lower in eyes with AMD compared with aged control eyes (analysis of variance, P=.003, P=.009, and P

Original languageEnglish (US)
Pages (from-to)670-678
Number of pages9
JournalArchives of Ophthalmology
Volume126
Issue number5
DOIs
StatePublished - May 2008

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Bruch Membrane
Angiogenesis Inhibitors
Macular Degeneration
Endostatins
Thrombospondin 1
Retinal Pigment Epithelium
Analysis of Variance

ASJC Scopus subject areas

  • Ophthalmology

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Reduction of endogenous angiogenesis inhibitors in bruch's membrane of the submacular region in eyes with age-related macular degeneration. / Bhutto, Imran A.; Uno, Koichi; Merges, Carol; Zhang, Lei; McLeod, D. Scott; Lutty, Gerard Anthony.

In: Archives of Ophthalmology, Vol. 126, No. 5, 05.2008, p. 670-678.

Research output: Contribution to journalArticle

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AU - Uno, Koichi

AU - Merges, Carol

AU - Zhang, Lei

AU - McLeod, D. Scott

AU - Lutty, Gerard Anthony

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