Reduction in polyamine catabolism leads to spermine-mediated airway epithelial injury and induces asthma features

V. Jain, S. Raina, A. P. Gheware, R. Singh, R. Rehman, V. Negi, Tracy Murray Stewart, U. Mabalirajan, A. K. Mishra, Robert A Casero, A. Agrawal, B. Ghosh

Research output: Contribution to journalArticle

Abstract

Background: Airway epithelial injury is a crucial component of acute and severe asthma pathogenesis and a promising target for treatment of refractory asthma. However, the underlying mechanism of epithelial injury remains poorly explored. Although high levels of polyamines, mainly spermine, have been found in asthma and comorbidity, their role in airway epithelial injury and the cause of their altered levels in asthma have not been explored. Methods: We measured key polyamine metabolic enzymes in lung samples from normal and asthmatic subjects and in mice with OVA-induced allergic airway inflammation (AAI). Polyamine metabolism was modulated using pharmacologic/genetic modulators. Epithelial stress and apoptosis were measured by TSLP levels and TUNEL assay, respectively. Results: We found loss of the polyamine catabolic enzymes spermidine/spermine-N (1)-acetyltransferase-1 (SAT1) and spermine oxidase (SMOX) predominantly in bronchial epithelial cells (BECs) of human asthmatic lung samples and mice with AAI. In naïve mice, SAT1 or SMOX knockdown led to airway hyper-responsiveness, remodeling, and BEC apoptosis. Conversely, in mice with AAI, overexpression of either SAT1 or SMOX alleviated asthmatic features and reduced TSLP levels and BEC apoptosis. Similarly, while pharmacological induction of SAT1 and SMOX using the polyamine analogue bis(ethyl)norspermine (BENSPM) alleviated asthmatic features with reduced TSLP levels and BEC apoptosis, pharmacological inhibition of these enzymes using BERENIL or MDL72527, respectively, worsened them. Spermine accumulation in lungs correlated with BEC apoptosis, and spermine treatment caused apoptosis of human BEAS-2B cells in vitro. Conclusions: Spermine induces BEC injury. Induction of polyamine catabolism may represent a novel therapeutic approach for asthma via reversing BEC stress.

Original languageEnglish (US)
Pages (from-to)2033-2045
Number of pages13
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume73
Issue number10
DOIs
StatePublished - Oct 1 2018

Fingerprint

Spermine
Polyamines
Asthma
Epithelial Cells
Apoptosis
Wounds and Injuries
Inflammation
Lung
Respiratory Hypersensitivity
Enzymes
Pharmacology
In Situ Nick-End Labeling
Comorbidity
polyamine oxidase

Keywords

  • asthma
  • catabolism
  • SAT1
  • SMOX
  • spermine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Reduction in polyamine catabolism leads to spermine-mediated airway epithelial injury and induces asthma features. / Jain, V.; Raina, S.; Gheware, A. P.; Singh, R.; Rehman, R.; Negi, V.; Murray Stewart, Tracy; Mabalirajan, U.; Mishra, A. K.; Casero, Robert A; Agrawal, A.; Ghosh, B.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 73, No. 10, 01.10.2018, p. 2033-2045.

Research output: Contribution to journalArticle

Jain, V. ; Raina, S. ; Gheware, A. P. ; Singh, R. ; Rehman, R. ; Negi, V. ; Murray Stewart, Tracy ; Mabalirajan, U. ; Mishra, A. K. ; Casero, Robert A ; Agrawal, A. ; Ghosh, B. / Reduction in polyamine catabolism leads to spermine-mediated airway epithelial injury and induces asthma features. In: Allergy: European Journal of Allergy and Clinical Immunology. 2018 ; Vol. 73, No. 10. pp. 2033-2045.
@article{b6a26ce506a44d678773e0decf77ef17,
title = "Reduction in polyamine catabolism leads to spermine-mediated airway epithelial injury and induces asthma features",
abstract = "Background: Airway epithelial injury is a crucial component of acute and severe asthma pathogenesis and a promising target for treatment of refractory asthma. However, the underlying mechanism of epithelial injury remains poorly explored. Although high levels of polyamines, mainly spermine, have been found in asthma and comorbidity, their role in airway epithelial injury and the cause of their altered levels in asthma have not been explored. Methods: We measured key polyamine metabolic enzymes in lung samples from normal and asthmatic subjects and in mice with OVA-induced allergic airway inflammation (AAI). Polyamine metabolism was modulated using pharmacologic/genetic modulators. Epithelial stress and apoptosis were measured by TSLP levels and TUNEL assay, respectively. Results: We found loss of the polyamine catabolic enzymes spermidine/spermine-N (1)-acetyltransferase-1 (SAT1) and spermine oxidase (SMOX) predominantly in bronchial epithelial cells (BECs) of human asthmatic lung samples and mice with AAI. In na{\"i}ve mice, SAT1 or SMOX knockdown led to airway hyper-responsiveness, remodeling, and BEC apoptosis. Conversely, in mice with AAI, overexpression of either SAT1 or SMOX alleviated asthmatic features and reduced TSLP levels and BEC apoptosis. Similarly, while pharmacological induction of SAT1 and SMOX using the polyamine analogue bis(ethyl)norspermine (BENSPM) alleviated asthmatic features with reduced TSLP levels and BEC apoptosis, pharmacological inhibition of these enzymes using BERENIL or MDL72527, respectively, worsened them. Spermine accumulation in lungs correlated with BEC apoptosis, and spermine treatment caused apoptosis of human BEAS-2B cells in vitro. Conclusions: Spermine induces BEC injury. Induction of polyamine catabolism may represent a novel therapeutic approach for asthma via reversing BEC stress.",
keywords = "asthma, catabolism, SAT1, SMOX, spermine",
author = "V. Jain and S. Raina and Gheware, {A. P.} and R. Singh and R. Rehman and V. Negi and {Murray Stewart}, Tracy and U. Mabalirajan and Mishra, {A. K.} and Casero, {Robert A} and A. Agrawal and B. Ghosh",
year = "2018",
month = "10",
day = "1",
doi = "10.1111/all.13472",
language = "English (US)",
volume = "73",
pages = "2033--2045",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - Reduction in polyamine catabolism leads to spermine-mediated airway epithelial injury and induces asthma features

AU - Jain, V.

AU - Raina, S.

AU - Gheware, A. P.

AU - Singh, R.

AU - Rehman, R.

AU - Negi, V.

AU - Murray Stewart, Tracy

AU - Mabalirajan, U.

AU - Mishra, A. K.

AU - Casero, Robert A

AU - Agrawal, A.

AU - Ghosh, B.

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Background: Airway epithelial injury is a crucial component of acute and severe asthma pathogenesis and a promising target for treatment of refractory asthma. However, the underlying mechanism of epithelial injury remains poorly explored. Although high levels of polyamines, mainly spermine, have been found in asthma and comorbidity, their role in airway epithelial injury and the cause of their altered levels in asthma have not been explored. Methods: We measured key polyamine metabolic enzymes in lung samples from normal and asthmatic subjects and in mice with OVA-induced allergic airway inflammation (AAI). Polyamine metabolism was modulated using pharmacologic/genetic modulators. Epithelial stress and apoptosis were measured by TSLP levels and TUNEL assay, respectively. Results: We found loss of the polyamine catabolic enzymes spermidine/spermine-N (1)-acetyltransferase-1 (SAT1) and spermine oxidase (SMOX) predominantly in bronchial epithelial cells (BECs) of human asthmatic lung samples and mice with AAI. In naïve mice, SAT1 or SMOX knockdown led to airway hyper-responsiveness, remodeling, and BEC apoptosis. Conversely, in mice with AAI, overexpression of either SAT1 or SMOX alleviated asthmatic features and reduced TSLP levels and BEC apoptosis. Similarly, while pharmacological induction of SAT1 and SMOX using the polyamine analogue bis(ethyl)norspermine (BENSPM) alleviated asthmatic features with reduced TSLP levels and BEC apoptosis, pharmacological inhibition of these enzymes using BERENIL or MDL72527, respectively, worsened them. Spermine accumulation in lungs correlated with BEC apoptosis, and spermine treatment caused apoptosis of human BEAS-2B cells in vitro. Conclusions: Spermine induces BEC injury. Induction of polyamine catabolism may represent a novel therapeutic approach for asthma via reversing BEC stress.

AB - Background: Airway epithelial injury is a crucial component of acute and severe asthma pathogenesis and a promising target for treatment of refractory asthma. However, the underlying mechanism of epithelial injury remains poorly explored. Although high levels of polyamines, mainly spermine, have been found in asthma and comorbidity, their role in airway epithelial injury and the cause of their altered levels in asthma have not been explored. Methods: We measured key polyamine metabolic enzymes in lung samples from normal and asthmatic subjects and in mice with OVA-induced allergic airway inflammation (AAI). Polyamine metabolism was modulated using pharmacologic/genetic modulators. Epithelial stress and apoptosis were measured by TSLP levels and TUNEL assay, respectively. Results: We found loss of the polyamine catabolic enzymes spermidine/spermine-N (1)-acetyltransferase-1 (SAT1) and spermine oxidase (SMOX) predominantly in bronchial epithelial cells (BECs) of human asthmatic lung samples and mice with AAI. In naïve mice, SAT1 or SMOX knockdown led to airway hyper-responsiveness, remodeling, and BEC apoptosis. Conversely, in mice with AAI, overexpression of either SAT1 or SMOX alleviated asthmatic features and reduced TSLP levels and BEC apoptosis. Similarly, while pharmacological induction of SAT1 and SMOX using the polyamine analogue bis(ethyl)norspermine (BENSPM) alleviated asthmatic features with reduced TSLP levels and BEC apoptosis, pharmacological inhibition of these enzymes using BERENIL or MDL72527, respectively, worsened them. Spermine accumulation in lungs correlated with BEC apoptosis, and spermine treatment caused apoptosis of human BEAS-2B cells in vitro. Conclusions: Spermine induces BEC injury. Induction of polyamine catabolism may represent a novel therapeutic approach for asthma via reversing BEC stress.

KW - asthma

KW - catabolism

KW - SAT1

KW - SMOX

KW - spermine

UR - http://www.scopus.com/inward/record.url?scp=85055639316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055639316&partnerID=8YFLogxK

U2 - 10.1111/all.13472

DO - 10.1111/all.13472

M3 - Article

C2 - 29729200

AN - SCOPUS:85055639316

VL - 73

SP - 2033

EP - 2045

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 10

ER -